Box-Behnken设计优化五指毛桃中多糖复合酶法的提取工艺  

Optimization of Enzymatic Extraction of Polysaccharides from Ficus simplicissima with Mixed Enzymes

作　　者：陈祝霞[1] 刘伟锐 赵荣军[1] 陈亮 钟鸣 CHEN Zhu-xia;LIU Wei-rui;ZHAO Rong-jun;CHEN Liang;ZHONG Ming(Dispensary of Taixing People's Hospital in Jiangsu Province,Taizhou 225400,China;Guangzhou Hanfang Pharmaceutical Corn- pang Litmited,Guangzhou 510240,China;National Engineering Research Center for Modernization of Extraction and Separation Process of TCM,Guangzhou 510970,China)

机构地区：[1]江苏省泰兴市人民医院药学科,江苏泰州225400 [2]广州白云山汉方现代药业有限公司,广东广州510240 [3]中药提取分离过程现代化国家工程研究中心,广东广州510970

出　　处：《中药材》2018年第2期400-406,共7页Journal of Chinese Medicinal Materials

基　　金：广州市科技计划项目(201503020011)

摘　　要：目的:优选混合酶法提取五指毛桃多糖的工艺。方法:通过单因素试验设计,以多糖收率为指标,考察不同影响因素对工艺的影响,并根据单因素结果通过D-最优混料设计优化复合酶的配比,用Box-Behnken试验设计法对得出的影响因素进行筛选。结果:混合酶最佳比例为果胶酶占25.2%、纤维素酶占66.7%、甘露聚糖酶占8.1%;混合酶法提取五指毛桃多糖最优工艺为加酶量5.0%、溶媒倍数36倍、反应体系p H 4.7、酶反应温度42.5℃及酶反应时间294 min,五指毛桃多糖收率在1.39%左右。结论:利用Box-Behnken效应面法优选的五指毛桃多糖复合酶提取工艺稳定可行,可用于五指毛桃多糖的提取。Objective:To optimize conditions of enzymatic extraction of Ficus simplicissima.Methods:Through the single factor experiment design,the effect of different factors on the process was investigated with the yield of polysaccharide as the index,optimization of compound enzyme ratio by D-optimal mixture design based on single factor results.Based on the response surface methodology by using Box-Behnken design,different influence factors were examined.Results:The optimal combination of enzymes were 25.2% pectinase,66.7% cellulase and 8.1% mannose.The dosage of the mixed enzyme was 5.0%,the solvent ratio was 36,initial reaction system pH was 4.7,enzymatic hydrolysis temperature was 42.5℃,and enzymatic hydrolysis time was 294 min.Under those conditions,the yield of polysaccharides of Ficus simplicissima was 1.39%.Conclusion:The Box-Behnken response surface methodology which used in the experiment to optimize the enzymatic extraction process for the polysaccharide of Ficus simplicissima is stable,effective and feasible.

关 键 词：五指毛桃 多糖 混合酶法 BOX-BEHNKEN效应面法 

分 类 号：R283.6[医药卫生—中药学]