生长抑素对糖尿病大鼠视网膜病变的神经保护作用及对GFAP、突触素表达的影响  

The neuroprotection of somatostatin and its effects on the expression of GFAP and synaptophysin in rats with diabetic retinopathy

作　　者：张博 李凤君[2] 左中夫 Zhang Bo;Li Fengjun;Zuo Zhongfu(Department of Ophthalmology,Huludao Ai'er Eye Hospital,Huludao 125001,China)

机构地区：[1]葫芦岛爱尔眼科医院综合眼科,125001 [2]唐山市眼科医院青光眼科,063000 [3]锦州医科大学解剖教研室,121001

出　　处：《国际内分泌代谢杂志》2018年第6期370-373,F0003,共5页International Journal of Endocrinology and Metabolism

基　　金：中国博士后科学基金项目(2017M612870);辽宁省高等学校杰出青年学者成长计划(LJQ2015069);辽宁省自然科学基金(201602340).

摘　　要：目的探讨生长抑素对糖尿病大鼠视网膜病变的神经保护作用及对神经胶质酸性蛋白(GFAP)、突触素表达的影响。方法清洁级雄性Sprague-Dawley(SD)大鼠24只,应用随机数字表法分成对照组、糖尿病组、生长抑素治疗组,每组8只。糖尿病组、生长抑素治疗组采用链脲佐菌素诱导糖尿病模型,模型诱导成功后,生长抑素治疗组给予腹腔注射鼠生长抑素(10μg·kg^-1·d^-1)。对照组、糖尿病组给予等剂量生理盐水。12周后,HE染色检测视网膜神经节细胞(RGC)密度,免疫荧光检测视网膜GFAP、突触素表达,Western印迹检测视网膜GFAP、突触素蛋白的相对表达量。结果与对照组相比,糖尿病组RGC密度下降,GFAP荧光强度升高,突触素荧光强度降低,GFAP蛋白表达升高,突触素表达降低(F=187.62、226.12、159.30、259.09、425.93,P均<0.01);而与糖尿病组相比,生长抑素治疗组RGC密度增加,GFAP荧光强度降低,突触素荧光强度升高,GFAP表达下降,突触素表达增加(F=187.62、226.12、159.30、259.09、425.93,P均<0.01)。结论生长抑素可能通过抑制胶质细胞活化,上调视网膜突触素表达,恢复视网膜RGC密度,对糖尿病视网膜病变具有神经保护作用。Objective To investigate the neuroprotective effects of somatostatin and its effects on the expression of glial fibrillary acidic protein (GFAP) and synaptophysin(SYN) in rats with diabetic retinopathy.Methods A total of 24 clean male Sprague-Dawley(SD) rats were divided into control group,diabetic group and somatostatin treatment group according to random number table,with 8 rats in each group.Diabetes was induced by streptozotocin in diabetic group and somatostatin treatment group.After diabetes was induced,rats in somatostatin treatment group were given somatostatin (10 μg · kg-1 · d-1) by intraperitoneal injection.Rats in control group and diabetic group were given the equal dose of saline.After 12 weeks,the density of retinal ganglion cells (RGC) were detected by HE staining;the expression of retinal GFAP and SYN were detected by immunofluorescence and Western blotting.Results Compared with control group,the fluorescence intensity of GFAP was significantly increased while the RGC density and the fluorescence intensity of SYN were decreased significantly.The expression of GFAP protein were increased and the expression of SYN protein was decreased in diabetic group compared with control group (F = 187.62,226.12,159.30,259.09,425.93,all P <0.01).Compared with diabetic group,the fluorescence intensity of GFAP was decreased significantly while the RGC density and the fluorescence intensity of SYN were increased significantly.The expression of GFAP was decreased and the expression of SYN was increased in somatostatin treatment group compared with diabetic group (F = 187.62,226.12,159.30,259.09,425.93,all P<0.01).Conclusion Somatostatin may increase the expression of SYN and restore the density of RGC by inhibiting the activation of glial cells,which suggests that somatostatin may play a neuroprotective role in diabetic retinopathy.

关 键 词：生长抑素 糖尿病视网膜病变 神经保护 神经胶质酸性蛋白 突触素 

分 类 号：R587.2[医药卫生—内分泌]