土荆芥挥发油对幽门螺杆菌甲硝唑耐药株及敏感株的体外抗菌作用研究  

Antibacterial activity of Chenopodium ambrosioides L. on Helicobacter pylori metronidazole resistant and susceptible strains in vitro

作　　者：于靖[1] 张学智[1] 史宗明[1] 黄秋月 叶晖[1] YU Jing;ZHANG Xue-zhi;SHI Zong-ming;HUANG Qiu-yue;YE Hui(Department of Integrated Traditional Chinese and Western Medicine,Peking University First Hospital,Beijing 100034,China)

机构地区：[1]北京大学第一医院中医中西医结合科,北京100034

出　　处：《北京中医药》2018年第10期929-931,共3页Beijing Journal of Traditional Chinese Medicine

基　　金：北京大学第一医院科研基金项目(2017QN37);国家自然科学基金项目(81473474)

摘　　要：目的研究土荆芥挥发油对幽门螺杆菌(HP)甲硝唑耐药株及敏感株的体外抗菌作用。方法选取HP甲硝唑耐药株(26695-16R、26695-8R16S、26695-2R8S)、敏感株(NCTC 11637、26695)作为实验菌株,采用倍比稀释法观察土荆芥挥发油对HP在不同浓度、不同作用时间下生长情况的影响,并测定其最低抑菌浓度(MIC)。结果土荆芥挥发油在不同时间情况下对HP甲硝唑耐药株及敏感株的MIC值为4～256μg/m L不等,其差异性随药物作用时间的延长而降低。结论土荆芥挥发油对HP甲硝唑耐药株及敏感株均具有明确的抗菌作用,其抗菌效能与药物剂量呈正相关,并于72 h后趋向稳定。Objective To study the bacteriostasis of Chenopodium ambrosioides L. against HP metronidazole resistant and susceptible strains in vitro. Methods The minimal inhibitory concentrations (MIC) of Chenopodium ambrosioides L. against HP were selected,agar dilution method used to observe the effect of the drug on the growth of HP under different drug concentrations and different action times,with HP metronidazole resistant strain (26695-16R,26695-8 R16S,26695-2 R8S) and susceptible strains (NCTC 11637,26695)setting as the experimental strains. Results Chenopodium ambrosioides L. was active against HP metronidazole resistant and susceptible strains under different action times (MIC value 4 ~ 256 μg/m L),and the differences of MIC values decreased with the prolongation of drug action time. Conclusion Chenopodium ambrosioides L. had bacteriostasis to HP metronidazole resistant and susceptible strains. The antibacterial effect was positively related with the drug concentrations and the effect tended to be stable after 72 h.

关 键 词：幽门螺杆菌 土荆芥挥发油 抗菌作用 

分 类 号：R259[医药卫生—中西医结合]