慢性阻塞性肺病大鼠“肺俞”微循环血流灌注量变化特征的研究  

Changes of the microcirculatory blood perfusion at “Feishu” (BL 13) in the COPD rats

作　　者：杨馥铭 汪天骅 张浩宇 周丹 徐枝芳 郭思佳[2] 高旸[3] 郭义[1] 郭永明[1] YANG Fuming;WANG Tianhua;ZHANG Haoyu;ZHOU Dan;XU Zhifang;GUO Sijia;GAO Yang;GUO Yi;GUO Yongming(Experimental Acupuncture Research Center of Tianjin University of TCM,Tianjin 300193,China;the Second Affiliated Hospital of Tianjin University of TCM,Tianjin 301617;the First Teaching Hospital of Tianjin University of TCM)

机构地区：[1]天津中医药大学实验针灸学研究中心,天津300193 [2]天津中医药大学第二附属医院,天津301617 [3]天津中医药大学第一附属医院

出　　处：《中国针灸》2018年第12期1303-1309,共7页Chinese Acupuncture & Moxibustion

基　　金：国家自然基金青年基金项目:81302916

摘　　要：目的:观察慢性阻塞性肺病(chronic obstructive pulmonary disease,COPD)大鼠的"肺俞"穴区微循环血流灌注量的特异性改变。方法:将60只Wistar大鼠按随机数字表法随机分为模型1组(C1组)、正常对照1组(N1组)、模型2组(C2组)和正常对照2组(N2组),每组15只。C1、C2组采用熏烟合并气管内滴内毒素方法造COPD模型,分别造模29 d、89 d;N1、N2组对应于C1、C2组同期给予正常饲养。选择"肺俞"、"心俞"、"肺俞"旁开、"心俞"旁开作为监测点,用激光散斑微循环血流监测系统(PeriCam PSI System,PSI)分别在造模前、造模29 d和89 d后监测上述监测点的微循环血流灌注量。结果:造模前4组大鼠各监测点微循环血流灌注量差异无统计学意义(均P>0.05),"肺俞""心俞"和其旁开对照点之间微循环血流灌注量差异无统计学意义(均P>0.05)。造模后,C1、C2组所有监测点微循环血流灌注量均升高(均P<0.05),C1组微循环血流灌注量高于N1组,C2组微循环血流灌注量低于N2组,C1组所有监测点微循环血流灌注量高于C2组,差异均有统计学意义(均P<0.05);C1、C2组出现了"肺俞"微循环血流灌注量高于旁开对照点的特异性变化,而N1、N2组没有出现。结论:COPD大鼠"肺俞"穴的微循环血流灌注量出现了与疾病病程相关的特异性变化。Objective To observe the change of the specificity of the microcirculatory blood perfusion at the area of “Feishu” (BL 13) in the rats of chronic obstructive pulmonary disease (COPD). Methods According to the random number table, 60 Wistar rats were divided into a 29 d model No. 1 group (C1 group), a 29 d normal control No.1 group (N1 group), a 89 d model No.2 group (C2 group) and a 89 d normal control No. 2 group (N2 group), 15 rats in each one. In the C1 and C2 groups, the smoking and intratracheal drops of endotoxin were used in combination to prepare COPD model. The rats were fed normally in the N1 and N2 groups. “Feishu” (BL 13), “Xinshu” (BL 15), the lateral site of “Feishu” (BL 13) and the lateral site of “Xinshu” (BL 15) were selected as the monitoring points. The pericam perfusion speckle imager (PeriCam PSI System) was adopted to monitor the microcirculatory perfusion unit (PU) at the monitoring points before and in 29 d and 89 d after modeling separately. Results Before modeling, the differences in PU were not significant at each monitoring point in comparison among the 4 groups and the differences were not significant among “Feishu” (BL 13) and “Xinshu” (BL 15) as well as their lateral sites (all P>0.05). After modeling, PU was increased at each monitoring point in the C1 and C2 groups (all P<0.05). PU in the C1 group was higher than the N1 group and that in the C2 group was lower than the N2 group, PU at each monitoring point in the C1 group were higher than the C2 group, indicating the significant differences (all P<0.05). In the C1 and C2 groups, the specific change occurred, in which PU at “Feishu” (BL 13) was higher than its lateral site. But such specific change did not happen in the N1 and N2 groups. Conclusion PU at “Feishu” (BL 13) presents the specific change relevant with the sickness duration in the COPD rats.

关 键 词：慢性阻塞性肺病(COPD) 穴 肺俞 微循环血流灌注量 内毒素 

分 类 号：R245[医药卫生—针灸推拿学]