金龟子绿僵菌FM-03生物学特性及其对柑橘粉蚧的侵染  

Biological Characteristics of Metarhizium anisopliae FM-03 and Its Infection against Planococcus citri

作　　者：黄鹏[1] 姚锦爱[1,2] 余德亿 HUANG Peng;YAO Jinai;YU Deyi(Institute of Plant Protection,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,Fuzhou 350003,China)

机构地区：[1]福建省农业科学院植物保护研究所,福州350013 [2]福建省作物有害生物监测与治理重点实验室,福州350003

出　　处：《中国生物防治学报》2018年第6期858-865,共8页Chinese Journal of Biological Control

基　　金：福建省省属公益类科研院所基本科研专项(2016R1023-8;2018R1025-2);福建省农业引导性(重点)项目(2017N0063);福建省农业科学院一般科研项目(AC2017-12);福建省农业科学院科技创新团队建设项目(STTT2017-2-2)

摘　　要：为明确金龟子绿僵菌FM-03菌株生物学特性及其对柑橘粉蚧的侵染效果,室内测定不同温度、pH、光周期、碳氮源对该菌株生长和产孢量的影响;观察柑橘粉蚧受该菌株侵染后的死亡趋势,构建该菌株对柑橘粉蚧的时间-剂量-死亡率(TDM)模型。结果表明,金龟子绿僵菌FM-03菌株培养最适的温度为25℃、pH 7、光周期12L:12D、碳源为乳糖、氮源为酵母膏,在此培养条件下菌落直径和产孢量分别可达6.17 cm和3.70×10~9孢子/皿。柑橘粉蚧受菌株FM-03侵染后的死亡趋势随菌株浓度升高和侵染时间延长而上升,1×10~8、1×10~7和1×10~6孢子/mL处理的升幅明显高于其他2个处理,其中在1×10~8孢子/mL处理的累计校正死亡率最高可达82.02%;构建的TDM模型,通过Pearson卡方和Hosmer-Lemeshow检验,显示菌株FM-03对柑橘粉蚧的剂量效应随侵染时间的延长逐渐增强,时间效应随浓度的升高逐渐增强,侵染8 d后的LC_(50)和1.00×10~8孢子/mL处理的LT_(50)分别可达6.03×10~5孢子/m L和4.53 d。综上,金龟子绿僵菌FM-03菌株生长快、产孢量高,对柑橘粉蚧的致病力强,生防应用前景良好。To definite the biological characteristics of Metarhizium anisopliae strain FM-03,the effects of temperature,pH,photoperiod,carbon source and nitrogen source on the mycelia growth and sporulation of FM-03strain were tested.Then the mortality of FM-03-infected Planococcus citri was observed,and the time-dosemortality model was constructed.Results showed that the optimal culture conditions for FM-03 growth and sporulation were 25℃,pH 7,12L:12D,with lactose as carbon source and yeast extract as nitrogen source.With these conditions,the colony diameter and sporulation quantity of FM-03 were 6.17 cm and 3.70×10^9 conidia/dish,respectively.The mortality of FM-03-infected P.citri increased with the increase in conidial concentration and the time of infection.The mortality increased more at 1×10^8,1×10^7 and 1×10^6 conidia/mL than at 1×10^5 and1×10^4 conidia/mL.The highest corrected accumulative mortality of P.citri reached 82.02%at 1×10^8 conidia/mL.Through Pearson’s chi-squared test and Hosmer-Lemeshow test,the TDM model of FM-03 against P.citri showed an enhanced dose effect of FM-03 against P.citri with the prolong of infection time,and an improved time effect with the increase in conidial concentration.The LC50 of FM-03 reached 6.03×10^5 conidia/mL after an eight-day infection.The LT50 of 1.00×10^8 conidia/mL reached 4.53 d.In conclusion,with fast growth,high sporulation quantity,and strong pathogenicity against P.citri,M.anisopliae FM-03 shows an excellent potential in biological control of the pest.

关 键 词：金龟子绿僵菌 生物学特性 柑橘粉蚧 时间-剂量-死亡率模型 

分 类 号：S433[农业科学—农业昆虫与害虫防治] S476.1[农业科学—植物保护]