二陈汤合桃红四物汤对ApoE基因敲除动脉粥样硬化小鼠作用及机制研究  

Effect and Mechanism of Er Chen Decoction and Taohong Siwu Decoction on ApoE Gene Knockout Atherosclerosis Mice

作　　者：王俊岩[1] 陈文娜[1] 贾连群[1] 张哲[1] 张林[1] 刘晶晶[1] 杨关林[1] WANG Jun-yan;CHEN Wen-na;JIA Lian-qun;ZHANG Zhe;ZHANG Lin;LIU Jing-jing;YANG Guan-lin(Liaoning University of Traditional Chinese Medicine,Shenyang,110847,China)

机构地区：[1]辽宁中医药大学,沈阳110847

出　　处：《中国中医基础医学杂志》2018年第11期1534-1536,1547,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家"973"基础发展计划(2013CB513704);国家自然科学基金资助项目(81300229);辽宁省高等学校优秀人才支持计划(LR2015041);辽宁中医药大学中医脏象理论及应用教育部重点实验室开放基金项目(zyzx1503;zyzx1602);沈阳市科技局产业发展应用基础研究计划(17-231-1-11)

摘　　要：目的:探讨二陈汤合桃红四物汤对ApoE基因敲除AS小鼠作用及作用机制。方法:50只ApoE-/-小鼠按随机数字表法分为模型组、中药低、中、高剂量组、阳性对照组,10只C57BL/6J小鼠作为空白对照组,模型组和各治疗组给予高脂饲料喂养,空白组给予普通饲料喂养,于第9周开始中药低、中、高剂量组、阳性对照组分别给予对应药物治疗4周,检测各组小鼠血脂水平、主动脉组织结构变化、血清TNF-α、IL-6、NO、ET-1水平变化、主动脉TGF-β、PI3K、AKT、eNOS蛋白水平变化。结果:与空白对照组比较,模型组小鼠主动脉有较大斑块形成,血清TC、TG、LDL、TNF-α、IL-6、ET-1水平显著升高,HDL、NO、NO/ET-1水平显著降低,主动脉TGF-β、PI3K蛋白表达水平、AKT、eNOS蛋白磷酸化水平显著降低。经治疗后各指标均有不同程度改善,以中药高剂量和阳性对照组效果较为明显。结论:二陈汤合桃红四物汤具有良好的抗AS的作用,其机制可能是通过激活TGF-β/PI3K/AKT/eNOS信号通路实现的。Objective: To investigate the effect and mechanism of Erchen Decoction and Taohong Siwu Decoction on ApoE-/-atherosclerosis mice. Method: 50 ApoE-/-mice were randomly divided into model group,low,medium and high dose groups,and positive control group,10 C57 BL/6 J mice served as a blank control group. The model group and the treatment group were fed with high fat diet,while the blank group was fed with regular diet. At the beginning of the ninth week,the low,medium and high dose groups and the positive control group were treated with corresponding drugs for 4 weeks. Serum lipid level,aortic tissue structure,levels of serum TNF-α,IL-6,NO and ET-1,levels of TGF-β,PI3 K,AKT,eNOS proteins were detected. Results: Compared with the blank control group,in the model group,the aorta had larger plaques,Serum levels of TC,TG,LDL,TNF-α,IL-6 and ET-1 were significantly increased,The levels of HDL,NO and NO/ET-1 decreased significantly. The levels of TGF-β,and PI3 K protein,the phosphorylation levels of AKT and eNOS proteins,were significantly decreased. After treatment,the indexes were improved in different degrees,and the effect was more obvious with high dose group and positive control group. Conclusion: Erchen decoction and Taohong Siwu Decoction has positive effect on AS. The mechanism may be achieved by activating the TGF-β/PI3 K/AKT/eNOS signaling pathway.

关 键 词：二陈汤 桃红四物汤 动脉粥样硬化 TGF-Β PI3K/AKT/eNOS 

分 类 号：R285.5[医药卫生—中药学]