2016-2017年青海省诺如病毒Ⅱ型基因型流行情况分析  

Analysis of the prevalence of norovirus genotypes in Qinghai Province from 2016 to 2017

作　　者：田登[1] 刘桂香[1] 徐琼[1] 赵生仓 张华一[1] 丰艳丽 肖敬玉 TIAN Deng;LIU Gui-xiang;XU Qiong;ZHAO Sheng-cang;ZHANG Hua-yi;FENG Yan-li;XIAO Jing-yu(Qinghai Province Center for Disease Control and Prevention,Xining,China 810007;Huangnan State Center for Disease Control and Prevention;Haixi State Center for Disease Control and Prevention)

机构地区：[1]青海省疾病预防控制中心,青海西宁810007 [2]黄南州疾病预防控制中心 [3]海西州疾病预防控制中心

出　　处：《中国病原生物学杂志》2018年第11期1258-1260,共3页Journal of Pathogen Biology

基　　金：国家科技重大专项传染病监测技术平台项目(No.2012ZX10004-208)

摘　　要：目的了解2016-2017年青海省诺如病毒Ⅱ型基因型及分子流行病学特点,为全省诺如病毒的防治提供理论基础和实验依据。方法用GⅡ型诺如病毒实时荧光PCR试剂盒检测GⅡ型诺如病毒核酸;用GⅡ型诺如病毒特异性引物COG2F/G2-SKR进行RT-PCR扩增,阳性产物回收纯化后直接测序,用Clustal和MEGA4.0生物软件对诺如病毒GⅡ基因序列进行比对和系统进化分析。结果不同地区来源的粪便标本238份,实时荧光PCR的方法检测GⅡ阳性标本47份,占19.74%。从47份阳性标本中分离到17株诺如病毒Ⅱ型核酸序列,包括诺如病毒Ⅱ型基因型GⅡ.1(4株)、GⅡ.2(8株)、GⅡ.3(2株)、GⅡ.4(2株)和GⅡ.14(1株),分别占23.53%、47.05%、11.76%、11.76%和5.88%。结论青海省流行的诺如病毒GⅡ型存在多种基因型,其中以诺如病毒GⅡ.2型为主,可为诺如病毒的防治提供参考。Objective To ascertain the genotype and molecular epidemiological characteristics of genogroup Ⅱ noroviruses isolated in Qinghai Province from 2016 to 2017 in order to provide a theoretical and experimental basis for the prevention and control the diseases caused by norovirus in Qinghai Province.Methods Norovirus genogroup Ⅱ (GⅡ) nucleic acids were detected using a real-time fluorescent PCR kit.Positive products were recovered and purified with RT-PCR using COG2F/G2-SKR for genogroup Ⅱ noroviruses.The gene sequences of genogroup Ⅱ noroviruses were analyzed using Clustal and MEGA 4.0.Results Of 238 stool specimens from different areas,47(19.74%)tested positive for geno-group Ⅱ noroviruses according to real-time fluorescent PCR.Seventeen norovirus genogroup Ⅱ strains were isolated from the 47 positive specimens.Nucleic acid sequences of those strains indicated that 4(23.53%) were norovirus genogroup Ⅱ genotype 1(GⅡ.1),8(47.05%) were genotype 2(GⅡ.2),2(11.76%) were genotype 3(GⅡ.3),2(11.76%)were genotype 4(GⅡ.4),and 1(5.88%) was genotype 14(GⅡ.14).Conclusion There are many genotypes of genogroup Ⅱ noroviruses in Qinghai Province,and norovirus genogroup Ⅱ genotype 2(GⅡ.2) is the predominant genotype.These findings can provide a reference for the prevention and treatment of norovirus infections.

关 键 词：诺如病毒Ⅱ型 基因型 流行分析 

分 类 号：R373.9[医药卫生—病原生物学]