基于SPR分析的金纳米粒子-适配体传感器检测梯恩梯  

Surface plasmon resonance analysis-based gold nanoparticle-aptamer sensor for the detection of 2,4,6-trinitrotoluene

作　　者：谭俊鹏 Natalia Pawlowska[1] 徐升晖 蔡能斌 杨瑞琴 郝红霞[1] TAN Jun-peng;Natalia Pawlowska;XU Sheng-hui;CAI Neng-bin;YANG Rui-qin;HAO Hong-xia(Key Laboratory of Evidence Science (China University of Political Science and Law),Ministry of Education,Collaborative Innovation Center of Judical Civilization China,Beijing 100088;People's Public Security University of China,Beijing 100038;Institute of Forensic Science,Zhangjiakou Municipal Public Security Bureau,Zhangjiakou 075000;Shanghai Key Laboratory of Criminal Scene Evidence,Shanghai 200083)

机构地区：[1]证据科学教育部重点实验室(中国政法大学),2011年计划司法文明协同创新中心,北京100088 [2]中国人民公安大学,北京100038 [3]张家口市公安局物证鉴定所,张家口075000 [4]上海市现场物证重点实验室,上海200083

出　　处：《分析试验室》2018年第12期1421-1425,共5页Chinese Journal of Analysis Laboratory

基　　金：中国政法大学青年教师学术创新团队资助项目(18CXTD09,16CXTD05);中国政法大学省部级合作项目(23317015);上海市现场物证重点实验室开放课题(2011-23417049)资助

摘　　要：构建了基于表面等离子体共振(SPR)分析的金纳米粒子(Au NPs)-TNT适配体传感器。该方法将TNT适配体运用在SPR检测方法中,并与Au NPs合成复合物以提高灵敏度。传感器芯片使用聚乙二醇结合2,4,6-三硝基苯基-甘氨酸进行修饰,将Au NPs-TNT适配体复合物作为配体,通过间接竞争法检测TNT。实验对溶液pH、进样时间、进样流速以及孵育时间进行优化。最优条件下(溶液pH 9.62、进样时间1500S、进样流速500μL/min、孵育时间30min),检测线性范围为0.5~20ng/mL,检出限58pg/mL(S/N=3)。该传感器可用于检测实际水样中TNT,在公共安全及环境监测领域具有良好的应用前景。A new surface plasmon resonance( SPR)-based gold nanoparticle( Au NPs)-TNT aptamer sensor was constructed. The sensitivity of the sensor was greatly improved because of formation of Au NPs-TNT aptamer complexes. The sensor chip was modified with polyethylene glycol which was combined with TNT analogue( 2,4,6-trinitrophenyl glycine). And Au NPs-TNT aptamer complexes were used as the ligand in the proposed sensor. TNT was detected by the indirect competition with TNT analogue. The experimental conditions,such as pH,injection time,sampling flow rate and incubation time were optimized. Under the optimal conditions( pH 9. 62,the sample time of 1500 s,the sampling flow rate of 500 μL/min,and the incubation time of 30 min),the inhibition ratio of sensor chip was linear with TNT concentration. The linear range of the proposed sensor was 0. 5 to 20 ng/m L. The limit of detection was 58 pg/m L( S/N = 3). The results demonstrated that the proposed sensor was suitable for TNT determination in complex water samples,and it had good application prospect in the field of public safety and environmental monitoring.

关 键 词：2 4 6-三硝基甲苯 表面等离子体共振 适配体 金纳米粒子 

分 类 号：X832[环境科学与工程—环境工程]