小麦GASA基因家族生物信息学分析  

Bioinformatics Identification of GASA Gene Family Expression Profiles in Wheat

作　　者：吕亮杰 陈希勇[1] 张业伦[1] 刘茜[1] 王莉梅[1] 马乐 李辉[1] Lu Liangjie;Chen Xiyong;Zhang Yelun;Liu Qian;Wang Limei;Ma Le;Li Hui(Hebei Academy of Agriculture and Forestry Sciences,Institute of Cereal and Oil Crops/Crop Genetics and Breeding Laboratory of Hebei,Shijiazhuang 050035,Hebei,China)

机构地区：[1]河北省农林科学院粮油作物研究所/河北省作物遗传育种实验室,河北石家庄050035

出　　处：《作物杂志》2018年第6期58-67,共10页Crops

基　　金：河北省现代农业科技创新工程项目(F18R01;494-0402-YBN-RDC4);河北省农林科学院财政专项(F17R0013;2018060303);河北省农林科学院粮油作物研究所开放课题(LYS2016004)

摘　　要：赤霉素调节的GASA(Gibberellic Acid-Stimulated in Arabidopsis)基因家族是植物特有的转录因子家族,在调控植物生长发育过程中起重要作用。关于小麦GASA基因家族全基因组分析的研究尚未见报道。为进一步探讨小麦GASA基因的功能,通过对小麦最新基因组数据进行分析,获得了35个TaGASA基因,命名为TaGASAs,根据染色体编号排列为TaGASA1～TaGASA35。结合公布的小麦品种Chinese Spring的基因组数据,采用生物信息学方法对其基因结构、染色体分布、蛋白结构、系统进化及表达谱进行了分析。结果表明,35个小麦TaGASA基因分布于除3A、4A、3B、3D染色体外的其余17条染色体上,基因编码长度为78～264个氨基酸的蛋白质,基因外显子数量从2个到7个不等;串联重复片段分析结果表明,片段复制和串联重复是小麦TaGASA家族基因扩张的主要模式;小麦TaGASA蛋白进化树和7种作物GASA基因的系统进化树表明,GASA基因分为4个类别,同一类之间的结构较为相似;小麦35个TaGASA基因家族成员含有10个motif,推测小麦TaGASA基因家族应都含有motif1、motif2和motif3。在13个组织器官中都检测到了35个TaGASA基因的转录本,不同组织器官中TaGASA基因的表达存在明显差异。The Gibberellic Acid-Stimulated in Arabidopsis(GASA) gene family is a specific transcription factor in the plant that plays an important role in the regulation of plant growth and development. However,genome-wide analysis of the GASA gene family has not been reported in wheat. To further explore the function of the wheat GASA gene, 35 TaGASA genes, named TaGASAs, were obtained by analyzing the latest genomic data of wheat and were ranked according to the chromosome number as TaGASA1-TaGASA35. Combined with the published genome data of cultivar Chinese Spring, genes structure, chromosome distribution, the conserved domain of proteins, phylogenetic trees and gene expression profiles of the wheat cultivars were analyzed using bioinformatics methods. The results showed that 35 wheat Ta GASA genes were distributed on the remaining 17 chromosomes except for 3A, 4A, 3B and 3D chromosomes. The genes encoded 78-264 amino acids in length and the number of gene exons was from 2 to 7. The results of tandem repeat analysis showed that fragment replication and tandem repeats were the main patterns of gene expansion in the wheat TaGASA family. The phylogenetic tree of wheat TaGASA proteins and the seven crops GASA proteins showed that GASA genes were divided into four categories, and the structure of the same class was similar. The 35 TaGASA genes family in wheat contain 10 motifs, and it is speculated that the wheat TaGASA gene family should contain motif1, motif2 and motif3. 35 TaGASA genes were all detected in 13 tissues and organs, and the expression of TaGASA genes in different tissues were significantly different.

关 键 词：小麦 GASA 生物信息学 进化树 表达谱 

分 类 号：S512.1[农业科学—作物学]