大叶藜指纹图谱研究及相似度分析  

Research of Fingerprint Spectrum and Similarity Analysis of Chenopodium Hybridum L.

作　　者：武芸[1] 王春林[2] 胡浩斌[1] 张腊腊[1] 韩明虎[1] WU Yun;WANG Chun-lin;HU Hao-bin;ZHANG La-la;HAN Ming-hu(College of Chemistry and Chemical Engineering,Longdong University,Qingyang 745000,China;College of Life Science and Technology,Longdong University,Qingyang 745000,China)

机构地区：[1]陇东学院化学化工学院,甘肃庆阳745000 [2]陇东学院生命科学与技术学院,甘肃庆阳745000

出　　处：《化学试剂》2018年第12期1165-1168,1215,共5页Chemical Reagents

基　　金：大叶藜化学成分及指纹图谱研究项目(ZJ201304)

摘　　要：通过大叶藜紫外指纹谱图(UVFP)、红外指纹谱图(IRFP),结合化学计量学方法,建立快速鉴别大叶藜药材的多维指纹图谱。采集10个不同产地的大叶藜样品190—700nm的UVFP和4000—400cm^-1的IRFP,分别反映中药化学组分中π→π^*、n→π^*、n→σ^*化学信息和多种化学单键、双键、三键等化学振动/转动对中红外线的吸收,建立其共有模式,并结合聚类分析和相似度分析构建多维指纹图谱。研究显示,UVFP、IRFP相结合构建的多维指纹图谱技术可实现对中药化学成分的全面检测。多维指纹图谱技术是一种综合鉴别中药质量的有效可行的方法,较任何单一方法更能准确揭示中药复杂系统的真实本源。An evaluation method of Chenopodium hybridum L. from different areas was established by adopting the ultraviolet fingerprints spectrum( UVFP) and infrared fingerprints spectrum( IRFP) and with the stoichiometry.UVFP( 190 ~ 700 nm) including the chemical information of the chemical constituents( π→π*,n→π*and n→σ*) and IRFP with the range of 4 000~400 cm-1 including the absorption capacity to mid-infrared of chemical bond vibration/rotation with single bond,double bond and triple bond were all recorded of Chenopodium hybridum L.. Samples from ten different areas of China established common pattern combining with the stoichiometry and similarity analysis to identify Chenopodium hybridum L..The combination of UVFP and IRFP achieved the overall monitoring of traditional Chinese medicine.The multidimensional fingerprints method was an effective and feasible method to comprehensively identify and objectively quantify traditional Chinese medicine quality,which can more accurately reflect the origin of the complex system of traditional Chinese medicine than any single method.

关 键 词：大叶藜 紫外指纹图谱 红外指纹图谱 相关系数 聚类分析 

分 类 号：R284.1[医药卫生—中药学]