十二指肠空肠旁路术对糖尿病大鼠肠道激素及肝脏内质网应激的影响  

Influence of duodenal-jejunal bypass surgery on intestinal hormone and hepatic endoplasmic reticulum stress in diabetic rats

作　　者：张鹏[1] 田鹏[1] 郭新宇 Zhang Peng;Tian Peng;Guo Xinyu(Department of General Surgery,Henan Provincial Peoples Hospital,Fuwai Central China Cardiovascular Hospital,Zhengzhou 450003,China)

机构地区：[1]河南省人民医院、阜外华中心血管病医院普外科,郑州450003

出　　处：《中华实验外科杂志》2018年第12期2275-2277,共3页Chinese Journal of Experimental Surgery

摘　　要：目的建立糖尿病大鼠模型,并实施十二指肠空肠旁路术(DJB),观察DJB对糖尿病大鼠肠道激素及肝脏内质网应激的影响。方法选购清洁级SD成年雄性大鼠58只,以高脂饮食喂养联合腹腔注射小剂量链脲佐菌素建立糖尿病大鼠模型,对建模成功的大鼠实施DJB(DJB组)及假手术(假手术组),以普通大鼠作为空白对照(空白对照组)。分别检测3组大鼠肠道激素、胰岛素。两组术后大鼠肝脏内质网应激指标[p-双链RNA依赖性蛋白激酶样内质网蛋白激酶(p-PERK)、肌醇需求激酶-1(IRE-1)、p-IRE-1]、肝脏组织中[总c-Jun氨基末端激酶(JNK)蛋白、p-JNK蛋白]、肝脏组织中胰岛素受体底物-1(IRS-1)蛋白以及p-IRS-1ser307蛋白的表达变化。结果灌胃前,3组大鼠胰高血糖素样肽-1(GLP-1)、抑胃肽(GIP)、胰岛素(INS)水平比较,差异无统计学意义(F=0.04、2.708、0.002,P=0.996、0.079、0.998);灌胃30min后,DJB组及对照组GLP-1、GIP、INS水平均高于假手术组,差异有统计学意义(F=33.692、18.657、16.393,P=0.000)。术后16周DJB组小鼠p-PERK、p-IRE-1、p-JNK、p-IRS-1ser307与术前比较,差异有统计学意义(t=10.507、12.606、9.163、14.603,P=0.000);IRE-1、JNK、IRS-1与术前比较,差异无统计学意义(t=1.295、1.863、1.675,P=0.205、0.072、0.104);相对于假手术组来说,DJB组改善良好。结论DJB能够有效改善高脂饮食喂养联合腹腔注射小剂量链脲佐菌素诱导的糖尿病大鼠肠道激素及胰岛素水平,缓解内质网应激。Objective To establish the diabetic rat model, to perform the duodenal-jejunal bypass surgery (DJB), and to observe the influence of DJB on intestinal hormone and hepatic endoplasmic reticulum stress in diabetic rats. Methods A total of 58 SD adult male rats induced by a high-fat diet feeding and intraperitoneal injection of low-dose streptozotocin to establish the diabetic rat model. Afterwards, DJB (DJB group) and sham surgery (sham surgery group) were conducted in the diabetic rats. The normal rats were taken as the blank control (blank control group). The intestinal hormone and insulin of three groups were detected. The changes of postoperative hepatic endoplasmic reticulum stress indexes [p-double stranded RNA-dependent protein kinase-like ER kinase (p-PERK), inositol requiring enzyme-1 (IRE-1), p-IRE-1], hepatic tissues [total c-Jun NH2-terminal kinase (JNK), p-JNK protein], insulin receptor substrate-1 (IRS-1) protein, p-IRS-1ser307 protein expression in hepatic tissues of two groups were observed. Results Before gastric perfusion, there was no statistical difference in the levels of glucagon-like peptide-1 (GLP-1), GIP, INS among the rats of three groups (F=0.04, 2.708, 0.002, P=0.996, 0.079, 0.998); After gastric perfusion for 30 min, the levels of GLP-1, GIP, INS in DJB group and control group were higher than those in sham surgery group (F=33.692, 18.657, 16.393, P=0.000); After surgery for 16 weeks, the p-PERK, p-IRE-1, p-JNK, p-IRS-1ser307 in DJB group were compared with those before surgery, and there was statistical difference (t=10.507, 12.606, 9.163, 14.603, P=0.000); The IRE-1, JNK, IRS-1 were compared with those before surgery, and there was no statistical difference (t=1.295, 1.863, 1.675, P=0.205, 0.072, 0.104); DJB group improved better than sham surgery group. Conclusion DJB for diabetic rats induced by high-fat diet feeding combined with intraperitoneal injection of low-dose streptozotocin can effectively improve intestinal hormone and insulin, relieve endoplasmic reticulum stress.

关 键 词：糖尿病 十二指肠空肠旁路术 大鼠肠道激素 肝脏内质网应激 

分 类 号：R587.1[医药卫生—内分泌]