四种抗生素对共培养的萱藻丝状体和膨胀色球藻的影响  

Inhibitory effect of four antibiotics on Chroococcus turgidus co-cultured to the filaments of Scytosiphon lomentaria

作　　者：张红霞 宫相忠[1] 高伟[1] 沈世军 王吉[1] 张静[1] ZHANG Hongxia;GONG Xiangzhong;GAO Wei;SHEN Shijun;WANG Ji;ZHANG Jing(College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China)

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《水产学报》2018年第12期1906-1915,共10页Journal of Fisheries of China

基　　金：国家"八六三"高技术研究发展计划(2012AA10A413);山东省重点研发计划(2016GSF115042)~~

摘　　要：为抑制或消除萱藻丝状体扩增阶段出现的膨胀色球藻,本研究探讨了头孢噻肟钠、阿莫西林、四环素和红霉素4种抗生素对共培养的萱藻丝状体和膨胀色球藻产生的影响。结果显示,浓度为50和100 mg/L的头孢噻肟钠均显著抑制膨胀色球藻的生长,进而保证萱藻丝状体正常生长与发育;浓度为50～1 000 mg/L的阿莫西林对膨胀色球藻和萱藻丝状体均无抑制作用,阿莫西林不适于去除共培养体系中的膨胀色球藻;浓度为100和200 mg/L的四环素对膨胀色球藻有显著的抑制作用,但此浓度下萱藻丝状体细胞质萎缩,生长状况较差;浓度为0.10～1.00 mg/L的红霉素对膨胀色球藻的生长均有显著的抑制作用,但当其浓度超过0.50 mg/L时,萱藻丝状体的生长亦受到抑制,甚至死亡。In order to suppress the growth of Chroococcus turgidus and finally eliminate it during the amplification process of filaments of Scytosiphon lomentaria,the effects of four antibiotics:cefotaxime sodium (CTX),amoxicillin (AM),tetracycline (TC) and erythromycin (EM) on the growth of filaments of S.lomentaria and C.turgidus,which were co-cultured in the research,were studied by experimental ecology methods in this paper.Results indicated that CTX can inhibit the growth of C.turgidus significantly at the concentration of 50 and 100 mg/L.On the 20th day,their average daily growth rates were 3.84% and -0.96%,respectively,which were significantly lower than 19.21% in the control group without antibiotics,therefore ensuring the normal growth and development of filaments of S.lomentaria.Both C.turgidus and the filaments of S.lomentaria were not suppressed by AM at the concentrations between 50 and 1 000 mg/L,so AM can not eliminate C.turgidus in the co-culture system.TC could inhibit the growth of C.turgidus and the filaments of S.lomentaria dramatically at the concentration of 100 and 200 mg/L,but the cytoplasm of filaments of S.lomentaria presented atrophy.EM inhibited the growth of C.turgidus significantly at the concentrations between 0.10 mg/L and 1.00 mg/L,when the concentration was higher than 0.50 mg/L,the growth of filaments of S.lomentaria also were inhibited,even to death.

关 键 词：萱藻 膨胀色球藻 丝状体 抗生素 共培养 

分 类 号：Q949.2[生物学—植物学] S968.4[农业科学—水产养殖]