机构地区:[1]空军军医大学唐都医院麻醉科,西安710038 [2]空军军医大学预防医学系放射医学研究室,西安710032
出 处:《神经解剖学杂志》2018年第6期710-714,共5页Chinese Journal of Neuroanatomy
基 金:国家自然科学基金(31570845);陕西省自然科学基础重大研究项目(2016ZDJC-16)
摘 要:目的:研究电磁脉冲(EMP)辐照造成大鼠脑组织损伤的机制,为防治EMP脑损伤提供新的理论依据。方法:成年雄性SD大鼠随机分为6组:对照组、EMP 30 min组、EMP1h组、EMP3h组、EMP6h组、EMP24h组。通过HE染色和Western Blot分析大鼠海马CA1区神经元经过EMP辐照处理后的形态变化,以及MyD88和JNK表达所受到的影响。本实验中EMP辐照系统的参数为:场强700kV/m,电压上升时间为3.5ns,脉宽为14ns,重复频率为1Hz,每次照射脉冲400p,每天照射1次,共照射3d。结果:(1)HE染色结果显示:经EMP辐照处理后,EMP1h组和EMP3h的海马CA1区异型细胞增多、细胞明显深染,形态不规则,细胞核形状多变,核仁不明显;EMP6h组和EMP24h组异型细胞形态改变,细胞形状扁平,细胞质常有空泡,细胞核分布偏移。(2)Western Blot结果显示:EMP辐照处理之后,EMP3h组的海马组织蛋白样品中,MyD88含量明显增高(P<0.05),于EMP6h组到达峰值,然后开始下降;EMP3h组中p-JNK的含量开始持续增高,EMP24h组中p-JNK的表达明显高于其余各组(P<0.05),并未出现下降趋势。结论:EMP辐照可以引起大鼠脑组织受损,这种损伤的机制可能是通过上调MyD88表达及促进JNK向p-JNK转化所引起,且两者不同的持续时间可能引起EMP造成脑损伤的主要机制随时间发生改变。Objective: To study the mechanism of electromagnetic pulse ( EMP) induced brain injury in rats and to provide a new theoretical basis for prevention and treatment of EMP injury.Methods: Adult male SD rats were randomly divided into six groups: control group,EMP 30 min group,EMP 1 h group,EMP 3 h group,EMP 6 h group,and EMP 24 h group.After EMP irradiation,the morphological change of neurons in the CA1 area of hippocampus were analyzed by HE staining and the expressive levels of myeloid differentiation factor 88 ( MyD88) ,p-JNK in neurons were measured by Western Blot.The parameters of the EMP irradiation system used in this experiment were: field strength 700 kV/m,voltage rise time 3.5 ns,pulse width 14 ns,repetition frequency 1 Hz,each irradiation pulse 400 p,irradiation once a day, total irradiation for 3 days.Results: ( 1) The results of HE staining showed that after EMP irradiation,in EMP 1 h group and EMP 3 h group,abnormal cells in the CA1 area of the hippocampus increased.The cells were significantly darkly stained,irregular in morphology,and varied in shape of the nucleus,with no obvious nucleoli.In EMP 6 h group and EMP 24 h group,the number of atypical cells decreased,the shape of cells was flat,the cytoplasm often had vacuoles, and the distribution of nuclei was deviated.( 2) Western Blot results showed that after EMP irradiation,MyD88 content in hippocampal tissue protein samples in the EMP 3 h group increased significantly ( P<0.05) .The peak reached at EMP 6 h group and then began to decline.The p-JNK content in the EMP 3 h group also began to increase continuously.The expression of p-JNK was significantly higher in the EMP 24 h group than in the other groups ( P<0.05) and there was no downward trend.Conclusion: EMP irradiation can cause the rat brain to be damaged.The mechanism of this damage may be caused by activation of the MyD88 and the JNK.The different duration of them may cause the main mechanism of brain damage caused by EMP changes with time.
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