基于转录组测序技术分析吡虫啉或氟啶虫胺腈对麦二叉蚜细胞色素P450基因表达的影响  

Effects of imidacloprid or sulfoxaflor on the expression of cytochrome P450 genes in greenbug(Schizaphis graminum Rondan) based on RNA sequencing

作　　者：张百重[1] 马康生[2] 刘俊杰 卢留洋 谢兰芬[1] 孔凡彬[1] 陈锡岭[1] 高希武[2] ZHANG Baizhong;MA Kangsneng;LIU Junjie;LU Liuyang;XIE Lanfen;KONG Fanbin;CHEN Xiling;GAO Xiwu(College of Resource and Environment,Henan Institute of Science and Technology,Xinxiang 453003,Henan Province,China;Department of Entomology,China Agricultural University,Beijing 100193,China)

机构地区：[1]河南科技学院资源与环境学院,河南新乡453003 [2]中国农业大学昆虫学系,北京100193

出　　处：《农药学学报》2018年第6期749-757,共9页Chinese Journal of Pesticide Science

基　　金：国家重点研发计划(2017YFD0201700);河南省科技攻关(农业)(182102110053);河南科技学院高层次人才引进启动项目(208010616003)

摘　　要：为了筛选出麦二叉蚜Schizaphis graminum参与吡虫啉或氟啶虫胺腈代谢调节的细胞色素P450(P450)基因,采用浸渍法,用吡虫啉或氟啶虫胺腈处理麦二叉蚜24 h后,通过构建转录组测序文库,并进行RNA-seq测序,筛选出差异性表达的P450基因,并进行实时荧光定量PCR(qPCR)验证。结果表明:用吡虫啉处理麦二叉蚜后,有19个P450基因上调表达,15个P450基因下调表达;用氟啶虫胺腈处理麦二叉蚜后,有18个P450基因上调表达,15个P450基因下调表达。运用qPCR验证了吡虫啉对12个P450基因的诱导表达,其结果与DGE测序文库显示的结果基本一致。这些结果可为深入研究麦二叉蚜P450对蚍虫啉和氟啶虫胺腈的解毒代谢作用提供依据。To identify the cytochrome P450(P450)genes involved in the metabolic regulation of imidacloprid or sulfoxaflor,the greenbug was treated with imidacloprid or sulfoxaflor for 24h using the dipping method.Then the differentially expressed P450genes were identified through transcriptome sequencing and quantitative real-time PCR (qPCR).The results indicated that the expression of 19P450 genes were up-regulated and 15P450genes were down-regulated in the greenbug treated with imidacloprid.In terms of the greenbug treated by sulfoxaflor,the expression of 18P450genes were up-regulated and 15P450genes were down-regulated.Furthermore,12P450genes affected by imidacloprid were validated by the qPCR method,and the results demonstrated that the expression levels of these genes were consistent with that of the DGE library.These results provide a basis for further study of the detoxification of imidacloprid and sulfoxaflor by P450s in the greenbug.

关 键 词：转录组测序 吡虫啉 氟啶虫胺腈 诱导 麦二叉蚜 细胞色素P450 基因表达 

分 类 号：Q965.9[生物学—昆虫学] S482.3[农业科学—农药学]