健脾通腑颗粒对肝郁脾虚型便秘型肠易激综合征大鼠模型的作用及机制研究  

Study on Effects of Jianpi Tongfu Granule on Constipation-predominant Irritable Bowel Syndrome Model Rats with Liver-stagnation and Spleen-deficiency and Its Mechanism

作　　者：刘媛[1] 唐洪梅[2] 钟如帆 陈玉兴 黄育生[2,4] 祝赫[1] 石玉莹[1] LIU Yuan;TANG Hongmei;ZHONG Rufan;CHEN yuxing;HUANG Yusheng;ZHU He;SHI Yuying(Guangzhou University of TCM,Guangzhou 510405,Guangdong,China;The First Affiliated Hospital of Guangzhou University of TCM,Guangzhou 510405,Guangdong,China;Guangdong Province Engineering Technology Research Institute of TCM Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine,Guangzhou 510095,Guangdong,China;Lingnan Medical Research Center of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong,China)

机构地区：[1]广州中医药大学,广东广州510405 [2]广州中医药大学第一附属医院,广东广州510405 [3]广东省中医药工程技术研究院,广东省中医药研究开发重点实验室,广东广州510095 [4]广州中医药大学岭南医学研究中心,广东广州510405

出　　处：《中华中医药学刊》2018年第12期2870-2874,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81673842,81073160);广东省自然科学基金项目(2014A030313404);广州市科技计划项目(201510010121)

摘　　要：目的:考察健脾通腑颗粒对肝郁脾虚便秘型肠易激综合征(IBS-C)大鼠模型的作用,并探讨其可能的作用机制。方法:取乳鼠10窝,随机留取一窝乳鼠作为正常对照组。其余9窝乳鼠采用母婴分离、冰水灌胃、限制饮水和低纤维饮食相结合的方法进行肝郁脾虚型IBS-C造模,将造模成功的大鼠随机分为模型对照组、枸橼酸莫沙必利组(2.7mg·kg^-1,临床等效剂量2倍)、复方芦荟胶囊组(0.31g·kg^-1,临床等效剂量2倍)、健脾通腑颗粒高、中、低剂量组(23.58,11.79,5.90g生药·kg^-1,临床等效剂量2、1、1/2倍)、逍遥丸组(3.24g·kg^-1,临床等效剂量2倍),每组10只。各给药组大鼠每天ig给予相应药物,正常对照组、模型对照组给予等量蒸馏水,连续14d。给药结束后测定各组大鼠的排便相关指标、肠道压力阈值、旷场实验相关指标、糖水偏好百分比、血清中5一羟色胺(5-HT)、生长抑素(SS)、P物质(SP)的含量、小肠推进率。结果:与模型对照组相比,健脾通腑颗粒能使肝郁脾虚型IBS-C大鼠的粪便粒数、粪便含水量、Bristol分值、肠道压力阈值、中央格停留时间、水平运动、糖水偏好百分比、小肠推进率明显增加(P<0.01或P<0.05),血清中5-HT、SS、SP的含量明显降低(P<0.0或P<0.01)。结论:健脾通腑颗粒可改善IBS-C模型大鼠的肝郁脾虚及便秘症状,其机制可能与降低血清中5-HT、SS、SP的含量有关。Objective :To observe the effects of Jianpi Tongfu Granule on constipation - predominant irritable bowel syndrome(IBS-C)model rats with liver-stagnation and spleen-deficiency and discuss its mechanism.Methods:A nest of neonatal rats were as the normal control group.The methods including maternal separation (MS),ice water stimulation,water limitation with low fiber diet were used to establish IBS -C model rats.The model rats were randomly divided into seven groups :model control group,mosapride group (2.7mg·kg^-1),Fufang Luhui Capsule (0.31g·kg^-1),Jianpi Tongfu Granule high ,medium and low-dose groups(23.58,11.79,5.90g drug·kg^-1),Xiaoyao Pills group(3.24g·kg^-1),with ten in each group.They were continuously administered by gavage for two weeks.The normal and model control group were given equivalent distilled water.After medication,the indexes of bowel movements in rats,intestinal pressure threshold,the indexes of open field test,the percentage of sugar preferences and small intestine advance rate were observed and the contents of 5-HT,SS and SP in serum were detected.Result:Compared with normal group,the defecation numbers and water content of the feces,and the percentage of sugar preferences,and the scores by Bristol stool scale were obviously decreased (P <0.01).Compared with model group,in each Jianpi Tongfu granule group,the defecation numbers and water content of the feces,the scores by Bristol stool scale,and the percentage of sugar preferences,intestinal pressure threshold,time of staying in center,horizontal displacement distance,small intestine advance rate were obviously increased (P <0.01or P <0.05),and the contents of 5-HT,SS and SP in serum were decreased (P <0.01or P <0.05).Conclusion :Jianpi Tongfu Granule can improve the symptoms of constipation in IBS-C with liver - stagnation and spleen - deficiency,which may be associated with decreasing the contents of 5-HT,SS and SP in serum.

关 键 词：便秘型肠易激综合征 肝郁脾虚 健脾通腑颗粒 

分 类 号：R285.5[医药卫生—中药学]