N,N,-偶联共轭双卟啉化合物与DNA的相互作用  

Study of N,N,-Conjugated Bisporphyrins Interacting with DNA

作　　者：刘丹 徐泽彬 王凯[1,2] 汪泽江[1] 吴风收 LIU Dan;XU Zebing;WANG Kai;WANG Zejiang;WU Fengshou(School of Chemical Engineering and Pharmacy,Wuhan Institute of Technology,Wuhan 430205,China;College of Chemistry and Chemical Engineering,Hubei University,Wuhan 430062,China)

机构地区：[1]武汉工程大学化工与制药学院,湖北武汉430205 [2]湖北大学化学化工学院,湖北武汉430062

出　　处：《武汉工程大学学报》2018年第6期591-596,共6页Journal of Wuhan Institute of Technology

基　　金：国家自然科学基金(20902071)

摘　　要：在我们先前的研究中,合成了三种新化合物,N,N-偶联四苯基双卟啉化合物(Por 1)及其Zn配合物(Por 2)和N,N-偶联甲基吡啶双卟啉化合物(Por 3)。通过紫外-可见吸收光谱、荧光发射光谱以及圆二色谱等方法,研究了这些卟啉化合物Por 1、Por 2和Por 3与ct-DNA的结合模式。研究结果表明:Por 1与DNA通过外部沟连和外部堆积模式结合。而Por 2与DNA则是外部沟连和插入模式结合,Por 3可以插入DNA之中,并且能借助于静电作用,与DNA的磷酸骨架进行结合。Por 1～Por 3与ct-DNA的结合常数分别为1.84×10~6L/mol、1.06×10~6L/mol和8.27×10~5L/mol。这些研究为后续的肿瘤细胞光动力活性测试奠定一定的理论基础。Three new compounds,N,N’-conjugated tetraphenyl bisporphyrin(Por 1),their Zn complex (Por 2)and N,N’-conjugated bisporphyrin with N-methyl pyridine(Por 3)were prepared in our previous studies. The properties of these compounds interacting with calf thymus DNA were investigated by UV-vis,fluorescence and circular dichroism spectroscopy in the present work. The results show that Por 1 interacts with ct-DNA through the groove binding and external stacking modes,while Por 2 binds to DNA with a combinationof external stacking with intercalation mode,and Por 3 can insert into DNA and integrate with its phosphate backbone through an electrostatic interaction. The binding constants of Por 1,Por 2 and Por 3 with ct-DNA arecalculated to be 1.84×10~6 L/mol,1.06×10~6 L/mol,and 8.27×10~5 L/mol,respectively. These studies provide a theoretical basis for the subsequent investigation of photodynamic activity tests against tumor cells.

关 键 词：卟啉 共轭双卟啉 CT-DNA 结合模式 相互作用 

分 类 号：O627[理学—有机化学] O614[理学—化学]