盐酸氯苯胍在异育银鲫体内的药代动力学研究  

The Pharmacokinetics of Robenidine hydrochloride in Crucian Carp(Carassius auratus gibelio)

作　　者：周宏正 赵燕楠 张祎桐 孙皓 童文涛 吕利群[1,2,3] ZHOU Hongzheng;ZHAO Yannan;ZHANG Yitong;SUN Hao;TONG Wentao;LV Liqun(National Pathogen Collection Center for Aquatic Animals,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Freshwater Aquatic Genetic Resources,Ministry of Agriculture,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学国家水生动物病原库,上海201306 [2]上海海洋大学农业部淡水水产种质资源重点实验室,上海201306 [3]上海海洋大学水产科学国家级实验教学示范中心,上海201306

出　　处：《上海海洋大学学报》2018年第6期916-923,共8页Journal of Shanghai Ocean University

基　　金：现代农业产业技术体系建设专项(CARS45-19).

摘　　要：盐酸氯苯胍是防治鲫孢子虫病的常用药。为了给鲫养殖过程中的临床科学用药提供理论依据,在水温为(25±1)℃时,分别用20、30和40 mg/kg的剂量口灌质量为(120±10) g的异育银鲫,利用高效液相色谱法测定ROBH在其肾脏、肝脏、血液、肌肉等4种组织的药时曲线。结果显示,3种剂量给药下,异育银鲫各组织中的ROBH药时曲线均呈双峰现象。不同剂量的药物在异育银鲫血液中的达峰时间、血药质量浓度峰值、药时曲线下面积分别为:20 mg/kg,T_(max)=2. 3 h,C_(max)=0. 55 mg/L,AUC=9. 35 [(mg/L)·h]; 30 mg/kg,T_(max)=2. 1 h,C_(max)=0. 73 mg/L,AUC=13. 02 [(mg/L)·h]; 40 mg/kg,T_(max)=2. 2 h,C_(max)=1. 17 mg/L,AUC=13. 98[(mg/L)·h]。给药剂量相同时,ROBH在异育银鲫各组织中的药时曲线下面积呈现肾脏、肝脏、血液、肌肉由大到小的排列顺序。清除速率显示,ROBH在肌肉中清除最慢,其次是血液。ROBH在肾脏和肝脏中具有一定蓄积作用,主要表现在药物含量高、MRT值偏大。本实验条件下,ROBH在异育银鲫体内的休药期不低于9 d。但考虑到实际养殖情况和天气原因,临床休药期可根据实际情况适当延长。本文系首次在大宗淡水鱼中报道ROBH的药代规律,为包括鲫在内的大宗淡水鱼养殖科学用药提供一定的理论指导。Robenidine hydrochloride(ROBH)is widely applied in treatment of diseases caused by sporozoan in freshwater aquaculture.In order to provide theoretical guidance for clinical rational administration in the breeding of crucian carp,pharmacokinetics of ROBH in Crucian Carp with body weight of(120±10)g was studied following oral administration of different doses (20mg/kg,30mg/kg and 40mg/kg)at water temperature of (25±1)℃.After administration,concentration-time cruves of ROBH in tested tissues of crueian carp exhibitied a "two-peak"pattern.The peak time ,the maximum concentration and the area under the concentration-time curve of three doses in blood were as follows :20mg/kg,Tmax =2.3h,Cmax=0.55 μg/ kg,AUC =9.35[(mg/L)·h] ;30mg/kg,Tmax =2.1h,Cmax =0.73mg/kg,AUC =13.02[(mg/L)·h] ; 40mg/kg,Tmax =2.2h,Cmax =1.17mg/kg,AUC =13.98[(mg/L)·h].Area under the concentrationtime curve value from higher to lower in tested tissues were:kidney,liver,blood and muscle.The lowest rate of elimination was found in muscle,which was followed by blood.The fact that high ROBH concentration and long MRT were identified in kidney and liver suggested some kind of accumulative effect in both of these two tissues.According to the pharmacokinetics of ROBH in muscle,we suggest a withdrawal period of no less than 9days in crucian carp.What's more,we suggest the withdrawal period can be adjusted properly because it might be influenced significantly by administration dose and temperature.This is the first pharmacokinetics report about ROBH in freshwater fish,which should be valuable in directing the application of ROBH for disease control.

关 键 词：异育银鲫 盐酸氯苯胍 药代动力学 黏孢子虫 

分 类 号：S948[农业科学—水产养殖]