免疫磁珠层析试纸法快速检测野生横纹东方鲀毒性  

Magnetic immunochromatographic method for rapid detection of toxicity inwild pufferfish( Takifugu oblongus)

作　　者：梁田田[1,2] 卢玉平 潘迎捷[1,2] 方国锋[1,2] 林昕 黄天娇[1,2] 卢瑛 LIANG Tiantian;LU Yuping;PAN Yingjie;FANG Guofeng;LIN Xin;HUANG Tianjiao;LU Ying(Shanghai Engineering Research Center of Aquatic-Product Processing &Preservation,College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Laboratory of Quality &Safety Risk Assessment for Aquatic Products on Storage and Preservation,Ministry of Agriculture,Shanghai 201306,China;Jiangsu Zhongyang Group Co.,Ltd,Haian 226000,Jiangsu,China)

机构地区：[1]上海海洋大学食品学院,上海水产品加工贮藏工程技术研究中心,上海201306 [2]农业部水产品贮藏保鲜质量安全风险评估实验室,上海201306 [3]江苏中洋集团股份有限公司,江苏海安226000

出　　处：《上海海洋大学学报》2018年第6期924-929,共6页Journal of Shanghai Ocean University

基　　金：上海市科学技术委员会部分地方院校能力建设项目(15320502100);上海市科学技术委员会工程研究中心推广项目(16DZ2280300).

摘　　要：调查并比较了野生横纹东方鲀(Takifugu oblongus)与养殖的菊黄东方鲀(Takifugu flavidus)、暗纹东方鲀(Takifugu obscurus)的毒性。随机选取17尾野生横纹东方鲀并用2%乙酸两次煮沸法提取各组织中河鲀毒素TTX,超滤离心去除杂蛋白,之后采用本实验室制备的免疫磁珠层析试纸条做定性检测,并对筛选出来的典型样本用国标小鼠生物法定量检测毒素含量与定性检测作对照,分析对比各组织TTX含量。野生横纹东方鲀肌肉和精巢TTX含量在0～7μg/g组织,由于个体差异处于无毒或弱毒水平;肝脏和卵巢TTX含量在4～400μg/g组织范围内,属于强毒水平;养殖菊黄东方鲀和暗纹东方鲀各组织均处于无毒水平。To provide some advice for people when they eat pufferfish,the toxicity of wild pufferfish( Takifugu oblongus Bloch) were investigated and compared with the toxicity of artificial feeding pufferfish( Takifugu flavidus; Takifugu obscurus). In this study,17 pufferfish tissues were randomly selected,next the tissue was extracted by boiling in 2% acetic acid. After centrifugation,the protein of supernatant was removed by ultrafiltration. The toxicity of 17 wild Takifugu oblongus samples was detected by magnetic immunochromatographic test strip prepared by our laboratory. The representative samples were analyzed and compared with mouse bioassay in regard to the toxicity. Our results showed that the toxicity of muscle and testicle was hypotoxicity,ranging between 0-7 μg/g because of individual difference; The toxicity of ovary and liver were in the range of 4-400 μg/g,was strongest toxicity; The tissues of cultured Fugu flavidus and Fugu obscures were non-toxic.

关 键 词：横纹东方鲀 毒性 TTX 小鼠生物法 免疫层析 

分 类 号：S948[农业科学—水产养殖]