ST3GAL4对MDA-MB-231增殖及c-met通路的影响探究  

Effects of ST3GAIA on proliferation and c-met signal pathway of MDA -MB -231

作　　者：高志康 李孝杰 于魁魁 谢凯[2] GAO Zhikang;LI Xiaojie;YU Kuikui;XIE Kai(Department of Cancer Vessel Intervention,the Affiliated Hospital of Xuzhou Medicine University,Xuzhou, Jiangsu 221002,China;Department of Clinical Laboratory,Xuzhou Central Hospital,Xuzhou,Jiangsu 221009)

机构地区：[1]徐州医科大学附属医院肿瘤血管介入科,江苏徐州221002 [2]徐州市中心医院检验科,江苏徐州221009

出　　处：《徐州医科大学学报》2018年第11期711-714,共4页Journal of Xuzhou Medical University

基　　金：江苏省资助博士后项目(2016259).

摘　　要：目的 探讨唾液酸转移酶ST3GAL4对人乳腺癌细胞系MDA-MB-231增殖和c-met通路的影响。方法 分别设计3段针对ST3GAL4编码区的siRNA,通过RT-PCR和Western blot两种方法分别对其mRNA水平和蛋白水平进行检测;接着通过敲减ST3GAL4观察MDA-MB-231平板克隆形成能力和c-met磷酸化水平。结果 向胞内转染siRNA 之后40小时,ST3GAL4的mRNA 水平和蛋白质水平较对照组(siNC)均有降低;相较于对照组,敲减组(si ST3GAL4) MDA-MB-231克隆形成能力明显减弱(p<0.05),c-met通路活化受到抑制。 结论 ST3GAL4可能参与MDA-MB-231的增殖与关键信号通路c-met的激活。objective To investigate the role of ST3GAL4 in proliferation and c-met signaling of human breast cancer cell line MDA-MB-231. Methods Three different siRNAs were designed to knock down ST3GAL4 according to its coding region. The expression of ST3GAL4 in mRNA and protein level was examined by RT-PCR and Western blot. Then colony formation assay and c-met phosphorylation of MDA-MB-231 were analyzed after knocking down of ST3GAL4. Results After transfection for 40 hours, the intracellular level of ST3GAL4 mRNA and protein were lower than those in control group (siNC). Ability of colony formation of MDA-MB-231 was significantly weakened, compared with control group (p<0.05). Moreover, activation of c-met pathway was inhibited. Conclusions ST3GAL4 may be involved in the proliferation and c-met signal pathway of MDA-MB-231.

关 键 词：ST3GAL4 MDA—MB一231 克隆形成 c—met 

分 类 号：Q291[生物学—细胞生物学]