诱导始期、温度和历期对松毛虫赤眼蜂滞育诱导及解除滞育的影响  

Effects of inducing initial stage, temperature and period on diapause induction and diapause termination of Trichogramma dendrolimi

作　　者：张俊杰[1] 张雪[1] 杜文梅[1] 阮长春[1] 臧连生[1] 任炳忠[2] Zhang Junjie;Zhang Xue;Du Wenmei;Ruan Changchun;Zang Liansheng;Ren Bingzhong(Engineering Research Center of Biological Control,Institute of Biological Control,Jilin Agricultural University,Changchun 130118,Jilin Province,China;School of Life Sciences,Northeast Normal University,Changchun 130024,Jilin Province,China)

机构地区：[1]吉林农业大学生物防治研究所,吉林省生物防治技术工程研究中心,长春130118 [2]东北师范大学生命科学院,长春130024

出　　处：《植物保护学报》2018年第6期1308-1313,共6页Journal of Plant Protection

基　　金：国家重点研发计划(2017YFD0200400);吉林省科技厅重点科技攻关计划(20160204010NY);吉林省科技发展计划项目(20170623002TC).

摘　　要：为实现赤眼蜂滞育技术在工厂化生产中的应用,在实验室条件下测定了诱导始期(卵期,幼虫前期、中期、后期,预蛹初期)、温度(8、10、12和14℃)及诱导历期(10、20、30和40 d)对松毛虫赤眼蜂Trichogramma dendrolimi滞育诱导的作用,并对其解除滞育的条件进行了探索。结果表明:松毛虫赤眼蜂从卵期到预蛹初期均可被诱导滞育,以幼虫中期诱导效果最佳。12℃为诱导松毛虫赤眼蜂滞育的最适温度,12℃下诱导滞育20 d,松毛虫赤眼蜂即可进入稳定的滞育状态,滞育率达97.4%。赤眼蜂滞育后,需经过一段时间的低温后才能完成滞育发育。滞育松毛虫赤眼蜂在3℃条件下储藏70 d,解除滞育率达95.9%,3℃条件下经历各储存时间的滞育解除率均高于0℃。In order to successfully utilize the diapause technology for the factory production of Trichogramma, the diapause induction conditions for T. dendrolimi were investigated in the laboratory. The effects of inducing initial developmental stage(egg, earlier, intermediate, and later periods of larvae, prepupae), inducing temperature(8, 10, 12 and 14℃) and inducing period(10, 20, 30, and 40 d) on diapause rate were investigated. Besides, the optimal conditions for diapause termination were also measured. The results showed that, from egg stage to prepupal stage, T. dendrolimi could be induced into diapause. On the whole, the intermediate larval stage was the most sensitive period for diapause induction. The suitable inducing constant temperature of T. dendrolimi was 12℃. At 12℃ for 20 days, 97.4%of T. dendrolimi were induced into diapause. Once the parasitoids entered diapause, they would experience a period of cold storage to complete the diapause development. At 3℃ for 70 days, the rate of diapause termination reached 95.9%. The diapause termination rates at 3℃ during all tested storage periods were higher than those stored at 0℃.

关 键 词：松毛虫赤眼蜂 滞育 恒温 诱导历期 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]