南葶苈子水提物对多柔比星诱导H9c2细胞凋亡和氧化应激的抑制作用  

Protective Effects of Aqueous Extract from Descurainia sophia on Doxorubicin-Induced Cardiomyocyte Injuryvia Suppressing Apoptosis and Oxidative Stress

作　　者：冯卫生[1,2] 杨方方 张莉[1,2] 杨雁芸[1] 白志尧 李孟 樊慧 栗萌萌[1] 郑晓珂 FENG Wei-sheng;YANG Fang-fang;ZHANG Li;YANG Yan-yun;BAI Zhi-yao;LI Meng;FAN Hui;LI Meng-meng;ZHENG Xiao-ke(Henan University of Chinese Medicine,Zhengzhou 450046,China;Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment &Chinese Medicine Development of Henan Province,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,郑州450046 [2]呼吸疾病诊疗与新药研发河南省协同创新中心,郑州450046

出　　处：《中国药学杂志》2018年第23期1999-2007,共9页Chinese Pharmaceutical Journal

基　　金：国家重点基础研究发展计划(973计划)项目资助(2013CB531802)

摘　　要：目的探明南葶苈子水提物对多柔比星(doxorubicine,Dox)引起的H9c2心肌细胞损伤的保护作用及潜在机制。方法应用5μg·m L^(-1)Dox处理H9c2细胞诱导建立心肌细胞损伤模型,分为对照组、模型组、阳性组、南葶苈子水提物不同剂量给药组,检测细胞活力,凋亡率,线粒体膜电位,细胞活性氧(ROS)、总超氧化物歧化酶(T-SOD)、乳酸脱氢酶(LDH)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)水平,凋亡通路关键蛋白p53、Bax、Bcl-2、Caspase-3蛋白表达水平,使用UPLC-MS联用的方法分析鉴定南葶苈子水提物中主要成分峰。结果南葶苈子水提物能提高H9c2细胞活力(P <0. 01),降低凋亡水平(P <0. 01),改善线粒体膜电位水平与ROS水平(P <0. 05),改善细胞内SOD及GSH-PX酶活力、LDH、MDA水平(P <0. 01或P <0. 05),调节凋亡通路关键蛋白caspase-3、Bax/Bcl-2、p53蛋白表达水平(P <0. 01或P <0. 05),同时,共分析鉴定了南葶苈子水提物中7种含量最高的成分。结论南葶苈子水提物可以有效保护由Dox引起的H9c2细胞损伤,其机制可能与改善细胞的氧化应激,抑制内源性凋亡通路,而抑制细胞凋亡的发生有关。OBJECTIVE To study the protective effects and mechanisms of aqueous extract from Descurainia sophia.on doxorubicin(Dox)-induced cardiomyocyte injury.METHODS The Dox induced H9c2 cell apoptotic model was established,then the cells were divided into normal group (NC),model group (Dox),positive group (resveratrol,RSV),and different doses of aqueous extract of Descurainia sophia (DS).The viability of H9c2 cells was detected by MTT assay.The apoptosis rate,mitochondrial membrane potential and reactive oxygen species (ROS)level were detected by flow cytometry.The levels of T-SOD,LDH,MDA and GSH-PX were measured.And the protein expression levels of caspase-3,Bcl-2,Bax and p53 were detected by western blot,HPLC-MS identification of aqueous extract from Descurainia sophia.RESULTS After treating with DS products,the survival rate of H9c2 was increased (P < 0.01),the apoptosis rate was significantly decreased (P <0.01),mitocbondrial membrane potential was significantly increased (P < 0.01),the level of ROS was significantly decreased (P <0.05),T-SOD and GSH-PX activities were significantly increased (P < 0.01),the levels of LDH and MDA content were significantly decreased(P <0.01).Moreover,DS reduced the expression of caspase-3 (P <0.01),regulated the expression of Bax/Bcl-2(P <0.01),decreased the expression of p53(P <0.05).Seven components were identified from DS by HPLC/MS analysis.CONCLUSION DS can effectively protect cardiomyocyte,and its mechanism is probably associated with correcting functional disorders of oxidative stress of cardiomyocytes,and inhibit mitochondrial apoptotic pathway.

关 键 词：南葶苈子 H9C2细胞 凋亡 氧化应激 

分 类 号：R965[医药卫生—药理学]