利用生物信息学工具分析“益气调血扶本培元”针法治疗老化痴呆的关键靶点  

Analysis ofKey Targets of “Yi Qi Tiao Xue Fu Ben Pei Yuan” Acupuncture in Treatment of Senile Dementia Using Bioinformatics Tools

作　　者：刘涛[1] 张雪竹[2] 贾玉洁[2] 韩景献[1] 聂坤[2] LIU Tao;ZHANG Xue-zhu;JIA Yu-jie;HAN Jing-xian;NIE Kun(Department of Acupuncture,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin (300193);Acupuncture Research Institute,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin (300193))

机构地区：[1]天津中医药大学第一附属医院针灸部,天津300193 [2]天津中医药大学第一附属医院针灸研究所,天津300193

出　　处：《中国中西医结合杂志》2018年第12期1474-1478,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81574049; No.81473766)

摘　　要：目的分析"益气调血扶本培元"针法治疗老化痴呆的关键靶点,阐明针刺治疗老化痴呆的细胞生物学机制。方法 60只7月龄快速老化痴呆SAMP8小鼠,随机分为对照组、针刺组和非穴组,每组各20只。针刺组以"益气调血扶本培元"针法针刺处理,非穴组处以非经非穴点针刺处理,对照组以单纯捉抓处理。采用Morris水迷宫方法观察各组小鼠学习记忆能力;提取各组小鼠海马组织的基因差异表达模式,利用GEO2Enrichr网络在线分析工具,分析差异表达基因的主要KEGG代谢网络途径,以及差异表达基因主要的细胞定位;提取各组小鼠海马组织的蛋白质组,利用DAVID在线注释工具,在蛋白质组水平分析各组小鼠海马组织差异表达蛋白质中线粒体相关蛋白质的数量。结果针刺组小鼠逃避潜伏期成绩从(79.90±14.82)s缩短到(28.64±12.71)s,较对照组和非穴组成绩显著缩短(P<0.01);针刺组和非穴组中均有多条代谢网络途径被活化,与非穴组比较,"氧化磷酸化"、"帕金森病"以及"蛋白质在内质网的加工"这3条代谢网络途径是针刺组特异的;针刺组表达上调的基因主要定位于神经元突起、线粒体和细胞骨架,非穴组的表达上调的基因主要定位于神经元突起;各组小鼠海马组织差异表达蛋白质中,表达上调的线粒体相关蛋白质数量,针刺组有32个,非穴组有5个。结论 "益气调血扶本培元"针法治疗老化痴呆的靶点很广泛,神经元线粒体是针刺疗效的关键靶点。Objective To analyze the key targets of "Yi Qi Tiao Xue Fu Ben Pei Yuan"(YQTXFBPY) acupuncture method in the treatment of senile dementia, and to elucidate the cellular biological mechanism of acupuncture in treatment of the disease. Methods As an animal model of senile dementia,7-month-old 60 senescence-accelerated mice prone 8(SAMP8)were randomly divided into the control group, the acupoint group and the non-acupoint group,with 20 in each group. Mice in the acupoint group were treated with YQTXFBPY acupuncture method. Mice in the non-acupoint group were acupunctured non-channel or non-acupoint. Mice in the control group were disposed with grasping alone. Morris water maze was used for analyzing the effect of acupuncture on the ability of learning and memory of SAMP8. Gene expression profiles of hippocampal tissue were extracted from each group. KEGG metabolic network pathways and cellular localization of differentially expressed genes were analyzed using GEO2Enrichr bioinformatics tools. Hippocampus tissue proteome were extracted from each group, and the numbers of mitochondria-associated proteins from each proteome were annotated using the DAVID online annotation tools. Results The escape latency of the acupoint group was shortened from(79.90±14.82)s seconds to(28.64±12.71)s, which was significantly shorter than that of the control and the non-acupoint group(P<0.01). There were multiple metabolic network pathways activated in both the acupoint group and the non-acupoint group. Compared with the non-acupoint group, the acupoint group had 3 specific metabolic pathways: "oxidative phosphorylation", "Parkinson′s disease" and "protein processing in endoplasmic reticulum". The up-regulated genes in the acupoint group were mainly located in neurite protuberance, mitochondria and cytoskeletons, while those in the non-acupuncture group were mainly located in neurite protuberance. In the proteome of mouse hippocampus, there were 32 up-regulated proteins related with mitochondria in acupoint group,while only 5 in non

关 键 词：老化痴呆 快速老年性小鼠 生物信息学 线粒体 

分 类 号：R245[医药卫生—针灸推拿学]