右美托咪定对脓毒症大鼠急性肾损伤的影响:与肾组织HMGB1 mRNA的关系  

Effect of dexmedetomidine on acute kidney injury in septic rats:the relationship with HMGB1mRNA in renal tissues

作　　者：叶艺峰[1] 胡淑辉[1] 陈彦青 王庆祥[1] Ye Yifeng;Hu Shuhui;Chert Yanqin;Wang Qingxiang(Department of Anesthesiology,First Affiliated Hospital of Xiamen University,Xiamen 361001,China;Department of Anesthesiology,Fujian Provincial Jinshan Hospital Teaching and Researching Unit of Anesthesiology Fujian Provincial Clinical College of Fujian Medical University,Fuzhou 350028,China)

机构地区：[1]厦门大学附属第一医院麻醉科,361001 [2]福建省立金山医院麻醉科福建医科、大学省立临床医学院麻醉教研室,福州市350028

出　　处：《中华麻醉学杂志》2018年第6期763-765,共3页Chinese Journal of Anesthesiology

摘　　要：目的 评价右美托咪定对脓毒症大鼠肾损伤的影响及其与肾组织高迁移率族蛋白B1(HMGB1) mRNA表达的关系.方法 健康成年雄性SD大鼠48只,8~12周龄,体重240~ 270 g,采用随机数表法分为3组(n=16):对照组(C组)、脓毒症组(S组)和右美托咪定组(D组).采用盲肠结扎穿孔法建立脓毒症模型.于术后24和48 h时采用免疫比浊法检测血清胱抑素C(Cys C)浓度.取血后放血处死大鼠,取肾组织,采用实时荧光定量PCR法检测HMGB1 mRNA的表达水平,观察病理学结果,行肾小管损伤评分.结果 与C组比较,S组和D组各时点肾小管损伤评分、血清Cys C浓度升高,肾组织HMGB1 mRNA表达上调(P<0.05);与S组比较,D组各时点肾小管损伤评分、血清Cys C浓度降低,肾组织HMGB1mRNA表达下调(P<0.05),肾脏组织病理学损伤减轻.结论 右美托眯定可减轻脓毒症大鼠急性肾损伤,机制可能与抑制肾组织HMGB1 mRNA表达有关.Objective To evaluate the effect of dexmedetomidine on acute kidney injury (AKI) and the relationship between the mechanism and expression of high mobility group protein B1(HMGB1) mRNA in renal tissues of septic rats.Methods Fort-eight healthy adult male Sprague-Dawley rats,aged 8-12weeks,weighing 240-270g,were divided into 3groups (n =16each)using a random number table method:control group (C group),sepsis group (S group )and dexmedetomidine group (D group).AKI was induced by cecal ligation and puncture.The concentrations of serum cystatin C (Cys C)were measured by immunoturbidimetry at 24and 48h after operation.The rats were sacrificed after blood sampling,and kidney tissues were removed for determination of the expression of HMGB 1mRNA (by fluorescent quantitative real-time polymerase chain reaction)and for examination of the pathological changes.The damage to the renal tubules was scored.Results Compared with group C,the renal tubular damage score and serum Cys C concentrations were significantly increased,arid the expression of HMGB1mRNA was up-regulated in S and D groups (P<0.05).Compared with group S,the renal tubular damage score and serum Cys C concentrations were significantly decreased,the expression of HMGB1mRNA was down-regulated (P<0.05), and the pathological changes of renal tissues were significantly attenuated in group D.Conclusion Dexmedetomidine can attenuate AKI in septic rats and the mechanism may be related to inhibiting the expression of HMGB1mRNA in renal tissues.

关 键 词：右美托咪啶 脓毒症 肾 高迁移率族蛋白质类 

分 类 号：R614[医药卫生—麻醉学]