花生下胚轴曝光与不曝光差异表达基因的数字表达谱分析  

Digital Gene Expression Profile Analysis of the Peanut Hypocotyl between under the Exposure and the Non-exposure

作　　者：刘冠明 林增顺 郑奕雄 徐庆国[1] Liu Guanming;Lin Zengshtm;Zheng Yixiong;Xu Qingguo(College of Agriculture,Hunan Agricultural University,Changsha,410000;College of Agriculture and Biology,Zhongkai University of Agriculture and Engineering,Guangzhou,510225)

机构地区：[1]湖南农业大学,农学院,长沙410000 [2]仲恺农业工程学院,农业与生物学院,广州510225

出　　处：《基因组学与应用生物学》2018年第12期5404-5413,共10页Genomics and Applied Biology

基　　金：广东省经济粮油作物产业技术体系创新团队育种与繁种岗位专家建设项目(2016LM1136)资助

摘　　要：为研究花生播种后通过覆土延长幼苗曝光,可促使下胚轴伸长,引伸子叶节出土,控制早花下针,有利于提高产量的机理,本研究采用数字基因表达谱的方法分析了花生幼苗曝光(处理)和不曝光(对照)下胚轴中的基因表达差异。结果表明,对照中检测到40275个基因,处理中检测到40554个基因;处理与对照相比,共检测出2722个表达差异基因,其中1887个基因上调表达,835个基因下调表达;根据GO分析标准,2722个表达差异基因可以分为3个本体类别。生物过程本体有22类功能的基因,其中代谢过程类有关的基因最多,其次是细胞过程类有关的基因;细胞组成本体有14类功能的基因,其中细胞和细胞组分类有关的基因最多,其次是细胞器类有关的基因;分子功能本体有12类功能的基因,其中催化类基因最多,其次是结合类基因。进一步对差异表达基因富积的植物激素代谢路径分析表明,生长素代谢路径中有6个差异表达基因,其中3个基因上调表达,3个基因下调表达;细胞分裂素代谢路径中有3个差异表达基因,全部为下调表达;赤霉素代谢路径中有3个差异表达基因,其中2个上调表达,1个下调表达;脱落酸代谢路径中有4个差异表达基因,其中3个表达上调,1个表达下调;乙烯代谢路径中有2个差异表达基因,1个上调表达,1个下调表达;茉莉酸代谢路径有9个差异表达基因,2个表达上调,7个表达下调;另外,油菜素甾醇代谢路径中有一个表达上调差异基因。本研究结果对了解延长幼苗曝光,促进下胚轴生长,控制下针有利于花生高产的机理具有重要意义。In order to further understand the mechanism of yield improvement through delaying the exposure time of the seedling by overlying soil after sowing, and then elongating the hypocotyls, bringing cotyledonary node up to ground, and controlling the early flowering and pegging, in this paper, genes expression differences in the hypocotyls of peanut seedling between under the exposure (treatment, T) and with the non-exposure (control, CK) have been analyzed with digital gene expression profile. The results showed that 40 275 unigenes were detected in control, and 40 554 genes were detected in the treatment. Compared to the control, 2 722 differentially expressed genes were detected in the treatment, of which 1 887 were up-regulated and 835 were down-regulated. According to GO international standardization, 2 722 expression differential genes were divided into three ontology categories (molecular function, cellular component and biological process). The ontology of biological process had 22 types of gene functions, which was the most genes related to the metabolic process, and then was the related genes of the cell process. There were 14 types of gene functions in the cellular component ontology, which was the most genes related to cell, followed by organelle related genes. There were 12 types of functional genes in the molecular functional ontology, including the most catalytic genes and the binging genes. The further analysis on the plant hormones metabolic pathways enriched different expression genes showed that there were 6 different expression genes in the auxin pathway, with 3 up and 3 down expression. The expressions of three genes were different in cytokinin pathway, which were down expression. In gibberellins metabolic pathway, three different expression genes were detected, of which two genes were up expression and one was down expression. There were 4 genes with different expression in abscisic acid metabolic pathway, of which three genes were up expression and one down expression. For ethylene metabolic pat

关 键 词：花生 下胚轴 光照 数字基因表达谱 基因表达差异 

分 类 号：S565.2[农业科学—作物学]