中药复方方剂对先兆流产大鼠妊娠结局及PGF2α、PPAP-A的影响  

Effects of compound traditional Chinese medicine on PGF2α and PAPP-A level in rats with threatened abortion

作　　者：游祥 王金玲 马晓宇 刘俊平 YOU Xiang;WANG Jinling;MA Xiaoyu;LIU Junping(Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,Ministry of Agriculture,P.R.China,Hohhot Inner Mongolia 010018,China)

机构地区：[1]农业部动物疾病临床诊疗技术重点实验室

出　　处：《中兽医医药杂志》2018年第6期5-8,共4页Journal of Traditional Chinese Veterinary Medicine

基　　金：国家重点研发计划“宠物疾病诊疗与防控新技术研究”(2016YFD0501000)

摘　　要：目的:通过研究不同剂量的复方中药对先兆流产大鼠前列腺素F2α(PGF2α)和妊娠相关蛋白A(PAPP-A)含量变化以及子宫组织的变化,探讨复方中药对妊娠大鼠药物导致的先兆流产与体内激素和子宫组织的相关关系。方法:以Wistar大鼠作为流产模型动物,选择怀孕7 d的大鼠,将其分为正常妊娠组、先兆流产模型组和治疗组,其中先兆流产模型组和治疗组用米非司酮联合米索前列醇进行造模,在观察到流产征兆后,治疗组分别以高、中、低剂量的复方中药水煎剂进行灌胃。在妊娠第11天,心脏采血处死大鼠,记录大鼠的活胚胎数和死胚胎数,ELISA检测大鼠血浆中PGF2α含量以及PAPP-A的含量。每只大鼠处死后取子宫制作石蜡切片,观察不同组子宫组织的变化。结果:灌服复方中药的大鼠PGF2α含量降低,子宫组织出血减少,PAPP-A含量升高,流产药物导致的妊娠大鼠胚胎流失数量相对减少。结论:该中药复方方剂可明显抑制大鼠前列腺素F2α的分泌和提高大鼠妊娠相关蛋白A水平,并且改善子宫内环境从而实现保胎作用。Objective:To examine the effects of PGF2α and PPAP-A level in rats with different does of compound traditional Chinese medicine,Wistar rats were selected as abortion model animal in the experiment. The 7-day pregnant rats were selected and divided into normal pregnancy group,threatened abortion model group and treatment group. Expected normal group,the other groups were used mifepristone combined with misoprostol to establish model of threatened abortion. After observing the symptoms of miscarriage the treatment group was given intragastric administration with high,medium and low doses of compound Chinese herbal decoction. On the 11 th day of gestation the rats were sacrificed by cardiac blood collection. The number of live fetuses and stillbirths were recorded. PGF2α content and PAPP-A content in rats plasma were detected by ELISA. After the rats were sacrificed uterus was taken to make paraffin sections and the changes of the uterus tissue were observed in different groups The results showed that content of PGF22α in rats fed with traditional Chinese herbal medicine was reduced,the hemorrhage in uterus decreased,the content of PAPP-A increased and the number of embryo loss in pregnant rats caused by miscarriage drugs decreased. Conclusion:The compound traditional Chinese medicine may prevent miscarriage by inhibiting the secretion of PGF2α and improving the intrauterine environment.

关 键 词：流产 中药 保胎 PGF2Α PAPP-A 

分 类 号：S853.74[农业科学—临床兽医学]