新风胶囊通过抑制PKC/NF-κB通路改善佐剂性关节炎大鼠的肺功能  

Xinfeng capsule improves pulmonary function of rats with rheumatoid arthritis by inhibiting PKC/NF-κB pathway

作　　者：万磊[1] 刘健[1] 黄传兵[1] 张晓军[2] 孙玥[1] 刘磊[1] WAN Lei;LIU Jian;HUANG Chuanbing;ZHANG Xiaojun;SUN Yue;LIU Lei(Department of Rheumatology and Immunology,First Affiliated Hospital,Hefei 230031;Clinical Medicine College,Anhui University of Chinese Medicine,Hefei 230038,China)

机构地区：[1]安徽中医药大学第一附属医院风湿免疫科,安徽合肥230031 [2]安徽中医药大学中医临床学院,安徽合肥230038

出　　处：《细胞与分子免疫学杂志》2018年第7期589-594,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81403388);安徽高校自然科学研究重点项目(KJ2017A281);安徽省自然科学基金(1508085QH159)

摘　　要：目的观察新风胶囊(XFC)对佐剂关节炎(AA)大鼠蛋白激酶C(PKC)/核因子κB (NF-κB)信号通路影响。方法将大鼠分正常对照(NC)组、模型对照(MC)组、XFC组、来氟米特(LEF)组。除NC组外,其余组采用Freund完全佐剂复制AA模型,第19天给药,每天1次,共30 d。XFC剂量为0. 34 g/(kg·d),1 m L/100 g灌胃,LEF剂量为0. 05 mg/(kg·d)灌胃;采用肺功能仪检测大鼠肺功能参数,ELISA检测血清白细胞介素6(IL-6)、IL-12、IL-10、IL-17、IL-35、基质金属蛋白酶9(MMP-9)水平,实时定量PCR检测肺组织Ras相关C3肉毒素底物1(Rac-1)、PKC、NF-κBp65 mRNA水平,Western blot法检测肺组织Rac-1、PKC、NF-κBp65蛋白水平,免疫组织化学染色法观察肺组织PKC、NF-κB的表达。结果 XFC组大鼠肺功能参数最大呼气第一秒呼出的气量的容积(FEV1)、50%肺活量的呼气流量(FEF50)、75%肺活量的呼气流量(FEF75)、用力最大呼气流量(PEF)较MC组显著升高,XFC组大鼠血清IL-10、IL-35水平升高,血清IL-6、IL-17、MMP-9降低,肺组织PKC、NF-κBp65、Rac-1 mRNA及PKC、NF-κBp65蛋白水平降低。结论 XFC通过抑制PKC/NF-κB通路,调节相关细胞因子的平衡,改善肺功能。Objective To observe the effect of Xinfeng capsule (XFC)on PKC/NF-κB pathway in the lung tissue of adjuvant arthritis (AA)rats.Methods Rats were divided into normal control(NC)group,model control(MC)group,XFC group,and leflunomide group (LEF).Except the NC group,the other three groups were induced into AA models with complete Freund's adjuvant (CFA).The XFC [0.34g/(kg·d),1 mL/100g)or LEF 0.05mg/(kg ·d)]was administrated from day 19 after the injection of CFA by gavage,once a day for 30 days.Pulmonary function was observed.The levels of IL-6,IL-12,IL-10,IL-17,IL-35 and matrix metalloproteinases (MMP)-9 were detected by ELISA.Ras-associated C3 botoxin substrate 1(Rac-1),PKC,NF-κBp65 mRNA were detected by real-time quantitative PCR.The levels of Rac-1,PKC, NF-κBp65 proteins were determined by Western blot analysis.The expression and distribution of PKC and NF-κB in the lung tissues were analyzed by immunohistochemical staining.Results The pulmonary function parameters such as FEV1, FEF50,FEF75 and peak expiratory flow (PEF)in the XFC group were significantly higher than those in the MC group.The expression of IL-10,IL-35 in the serum increased,and IL-6,IL-17 and MMP-9 decreased.Compared with the MC group,the expression of PKC,NF-κBp65 and Rac-1 decreased in the XFC group.Conclusion XFC improves the lung function by inhibiting the PKC/NF-κB pathway and balancing the cytokine network.

关 键 词：佐剂性关节炎 肺功能 蛋白激酶C(PKC) 核因子κB(NF-κB) 细胞因子网络 新风胶囊 

分 类 号：R453.9[医药卫生—治疗学] R593.2[医药卫生—临床医学]