β七叶皂苷(β-aescin)通过抑制脂质过氧化及炎症反应减轻脂多糖诱导的小鼠急性肺损伤  

β-aescin alleviates acute lung injury induced by lipopolysaccharide by inhibiting lipid peroxidation and inflammation in mice

作　　者：王保健[1] 毛旭 朱建伟[3] WANG Baojian;MAO Xu;ZHU Jianwei(Department of Respiration,the 153Hospital of People's Liberation Army,Zhengzhou 450042;Department of Oral and Maxillofaeial Surgery,Sehool of Stomatology,Fourth Military Medical University,Xi 'an710032;Department of Laboratory Medicine,Xi 'an Central Hospital,Xi'an Jiaotong University,Xi'an 710003,China)

机构地区：[1]中国人民解放军153医院呼吸科,河南郑州450042 [2]空军军医大学口腔医院颌面外科,陕西西安710032 [3]西安市中心医院检验科,陕西西安710003

出　　处：《细胞与分子免疫学杂志》2018年第7期600-604,共5页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的研究β七叶皂苷(β-aescin)对脂多糖诱导的急性肺损伤(LPS-ALI)的保护作用及机制。方法雄性C57BL/6小鼠60只随机分为正常对照组、β-aescin处理组、LPS-ALI模型组、β-aescin预处理的LPS-ALI组,每组15只。LPS-ALI模型组小鼠采用水合氯醛腹腔注射麻醉,按10 mg/kg剂量经口咽插管匀速将LPS注入肺中;β-aescin预处理的LPS-ALI组于LPS注射前0. 5 h按1 mg/kg剂量腹腔注射β-aescin;正常对照组、β-aescin处理组经腹腔注射等量PBS或β-aescin。各组动物在模型制备后0. 5、2、4、6、8 h,取1 m L腹主动脉血进行血气分析测定氧分压(Pa O2)、二氧化碳分压(Pa CO2); 3 d后多聚甲醛灌注,取材行冰冻切片,HE染色观察肺组织病理改变; 3 d后取1/3肺脏进行测定干/湿质量比(D/W),1/3肺脏采用商品化试剂盒检测髓过氧化物酶(MPO)及丙二醛(MDA)水平;其余肺组织采用实时荧光定量PCR检测促炎因子肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)及IL-6的mRNA水平。结果β-aescin可显著减轻LPS诱导的肺组织病变;增加Pa O2而降低Pa CO2;增加肺组织D/W比例;降低脂质过氧化相关指标MPO及MDA的水平,并下调TNF-α、IL-1β及IL-6的mRNA水平。结论β-aescin通过抑制脂质过氧化反应及炎症反应显著减轻LPS-ALI后肺组织病变,并改善肺脏换气功能。Objective To study the protective effect of β-aescin on lipopolysaccharide (LPS)induced-acute lung injury (LPS-ALI)and to explore the underlying mechanism.Methods Sixty male C57BL/6 mice were randomly divided into four groups including normal control group,β-aescin-treated group,LPS-ALI group,LPS-ALI combined with β-aescin-treated group,with 15 mice in each group.For LPS-ALI group,the mice were intraperitoneally injected with chloral hydrate and 10mg/kg of LPS was then injected into the lungs through oropharyngeal intubation.For LPS-ALI combined with β-aescintreated group,β-aescin(1mg/kg)was intraperitoneally injected 0.5 hour before LPS injection.For normal control group or β-aescin control group,the same amount of PBS or β-aescin was intraperitoneally injected.Blood gas analysis of 1mL blood taken from abdominal aorta was performed at 0.5,2,4,6 and 8 hours after different treatments.Lungs were obtained at 3 days after different treatment for frozen sections preparation,HE staining,dry/wet mass (D/W) ratio,detection of MPO and MDA,and examination of TNF-α,IL-6 and IL-1β expression.Results β-aescin can significantly reduce the pathological changes of lung tissue,lower PaCO2 while increase PaO2and D/W ratio,down-regulate the expression of TNF-α,IL-1β and IL-6 in LPS-ALI mice.Conclusion β-aescin can significantly reduce the degree of lung injury and improve function of gas exchange in LPS-ALI mice by inhibiting lipid peroxidation production and expression of pro-inflammatory factors.

关 键 词：急性肺损伤(ALI) β七叶皂苷(β-aescin) 脂质过氧化 炎症 

分 类 号：R392.11[医药卫生—免疫学] R364.5[医药卫生—基础医学]