外伤性癫痫灶β-折叠蛋白的FTIR-mapping研究  

A study on detection of β-sheet protein in posttraumatic epileptogenic focus by FTIR-mapping

作　　者：向思阳 姜竹青[1,2] 李江涛[1,2] 尤萌[1,2] 张海东[1,2] 王旭[1,2] 杨天潼[1,2] Xiang Siyang;Jiang Zhuqing;Li Jiangtao;You Meng;Zhang Haidong;Wang Xu;Yang Tiantong(Center of Cooperative Innovation for Judicial Civilization,China University of Political Science and Law &Jilin University &Wuhan University,Beijing 100088,China;China University of Political Science and Law,Key Laboratory of Evidence Science,Ministry ofEducation,Beijing 100088,China)

机构地区：[1]中国政法大学吉林大学武汉大学司法文明协同创新中心,北京100088 [2]中国政法大学教育部证据科学重点实验室,北京100088

出　　处：《中国法医学杂志》2018年第6期578-581,共4页Chinese Journal of Forensic Medicine

基　　金：北京市优秀人才培养资助项目(2013D002023000002);中国政法大学青年教师学术创新团队资助项目(2016CXTD05;2014CXTD04)

摘　　要：目的研究外伤性癫痫致痫灶β-折叠蛋白的红外光谱改变。方法利用傅里叶红外光谱面扫描成像技术对大鼠外伤性癫痫脑组织标本进行检测,获得β-折叠蛋白结构的光谱数据,绘制致痫灶的红外光谱图像。结果致痫灶可见明显的β-折叠蛋白结构高吸收区,高吸收区色差、吸收度与低吸收区存在明显不同。结论外伤性癫痫致痫灶广泛存在β-折叠结构跨膜蛋白的改变,具有β-折叠结构的跨膜蛋白广泛参与了外伤性癫痫的发生。Objective The article is a study on detection of β-sheet protein in posttraumatic epileptogenic focus by FTIR-mapping.Methods Fourier transform infrared spectroscopy and fourier transform infrared spectroscopy-mapping were applied to identifying β-sheet through point-by-point scanning in posttraumatic epileptogenic focus sections and to developing FTIR-mapping profiles.Result The high absorbance of β-sheet is in accord with posttraumatic epilepsy,there is significant difference between high absorbance and low absorbance.Conclusion β-sheet proteins are distributed in posttraumatic epileptogenic focus widely.β-sheet proteins are involved in posttraumatic epilepsy.

关 键 词：法医学 外伤性癫痫 傅里叶红外光谱 β-折叠结构 

分 类 号：DF795.1[医药卫生—法医学]