Generation and functional characterization of a conditional Pumilio2 null allele  

作　　者：Kaibo Lin Shikun Zhang Jieli Chen Ding Yang Mengyi Zhu Eugene Yujun Xu 

机构地区：[1]State Key Laboratory of Reproductive Medicine, Nanjing Medical University

出　　处：《The Journal of Biomedical Research》2018年第6期434-441,共8页生物医学研究杂志（英文版）

基　　金：supported by National Basic Research Program of China(973 program,2013CB945201 and 2015CB-943002);National Science Foundation of China(81270737);Natural Science Foundation of Jiangsu Province(BK2012838);Provincial Innovation and Entrepreneurship Grant as well as NIH grant U01HD045871;Provincial Shuangchuang Program

摘　　要：The highly conserved RNA binding protein PUF(Pumilio/FBF) family is present throughout eukaryotes from yeast to mammals, with critical roles in development, fertility and the nervous system. However, the function of the mammalian PUF family members remains underexplored. Our previous study reported that a gene-trap mutation of Purm2 results in a smaller testis but does not impact fertility and viability. Although the gene-trap mutation disrupted the key functional domain of PUM protein-PUM-HD(Pumilio homology domain), but still produced a chimeric Pum2-β-geo protein containing part of PUM2, raising a question if such a chimeric protein may provide any residual function or contribute to the reproductive phenotype. Here, we report the generation of a conditional PUM2 allele,when knocked out, producing no residual PUM2 and hence a complete loss-of-function allele. We also uncovered small but significant reduction of male fertility and viability in the mutants, suggesting requirement of PUM2 for male fertility and viability.The highly conserved RNA binding protein PUF(Pumilio/FBF) family is present throughout eukaryotes from yeast to mammals, with critical roles in development, fertility and the nervous system. However, the function of the mammalian PUF family members remains underexplored. Our previous study reported that a gene-trap mutation of Purm2 results in a smaller testis but does not impact fertility and viability. Although the gene-trap mutation disrupted the key functional domain of PUM protein-PUM-HD(Pumilio homology domain), but still produced a chimeric Pum2-β-geo protein containing part of PUM2, raising a question if such a chimeric protein may provide any residual function or contribute to the reproductive phenotype. Here, we report the generation of a conditional PUM2 allele,when knocked out, producing no residual PUM2 and hence a complete loss-of-function allele. We also uncovered small but significant reduction of male fertility and viability in the mutants, suggesting requirement of PUM2 for male fertility and viability.

关 键 词：RNA-binding protein Pumilio2 PUF FERTILITY VIABILITY 

分 类 号：R339.21[医药卫生—人体生理学]