葱白提取物对非酒精性脂肪肝模型大鼠PGC-1α和PEPCK,G6Pase表达的影响  

Effect of Allii Fistulosi Bulbus Extract on Expression of PGC-1α,PEPCK and G6Pase in Rat Models with Non-alcohol Fatty Liver Disease

作　　者：郑丁 时昭红[2] 郭洁[2] 张书[2] 付丽鹤 刘凡[1] 涂蓓蕾 ZHENG Ding;SHI Zhao-hong;GUO Jie;ZHANG Shu;FU Li-he;LIU Fan;TU Bei-lei(Hubei University of Chinese Medicine,Wuhan 430055,China;Wuhan Hospital of Traditional Chinese and Western Medicine,Wuhan 430022,China)

机构地区：[1]湖北中医药大学,武汉430065 [2]武汉市中西医结合医院,武汉430022

出　　处：《中国实验方剂学杂志》2018年第24期128-133,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173217);国家自然科学基金青年基金项目(81202676)

摘　　要：目的:观察葱白提取物对非酒精性脂肪肝(NAFLD)模型大鼠过氧化物酶体增殖物激活受体γ辅助激活因子-1α(PGC-1α),磷酸烯醇式丙酮酸羧激酶(PEPCK),葡萄糖6磷酸酶(G6Pase)表达的影响,并探讨其作用机制。方法:采用高脂饲料复制非酒精性脂肪肝大鼠模型,并应用小干扰RNA(siRNA)技术抑制PGC-1α的表达,将60只大鼠随机分为正常组、模型组、葱白组(50 mg·kg-1)、转染组、空转组、葱白加转染组。苏木素-伊红(HE)染色观察肝组织组织结构;检测血液流变学及血清天门冬氨酸氨基转移酶(AST),丙氨酸氨基转移酶(ALT),总胆固醇(TC),甘油三酯(TG)水平;实时荧光定量聚合酶链式反应(Real-time PCR)与蛋白免疫印迹法(Western blot)检测大鼠肝脏PGC-1α,PEPCK,G6Pase mRNA和蛋白的表达。结果:与正常组比较,模型组大鼠肝脏出现明显脂肪变性;大鼠血浆黏度(低切、中切、高切)、全血黏度均不同程度升高(P <0. 05,P <0. 01);血清ALT,AST,TC,TG水平明显增高(P <0. 05); PGC-1α,PEPCK,G6Pase mRNA表达水平明显降低(P <0. 05);给予葱白提取物干预后,葱白组大鼠肝脂肪变性明显减轻;血清ALT,AST,TC,TG水平均降低(P <0. 05),PGC-1α,PEPCK,G6Pase mRNA和蛋白表达水平升高(P <0. 05)。PGC-1α转染的大鼠,即使给予同等剂量葱白提取物,与模型组比较,葱白加转染组肝脂肪变性未见明显改善;葱白加转染组PGC-1α,PEPCK,G6Pase mRNA表达无明显差异。结论:葱白提取物能够改善NAFLD模型大鼠肝脂肪变性,其机制可能与增加PGC-1α的转录活性,促进PEPCK,G6Pase的表达进而增加线粒体合成有关。Objective:To observe the effect of Allii Fistulosi Bulbus extract on the expression of peroxisome proliferator activated receptor-y coactivator l a (PGC-lα),phosphoenolpyruvate carboxykinase (PEPCK)and glucose-6-phosphatase (G6Pase)in rat models with non-alcoholic fatty liver disease (NAFLD), and explore its possible mechanism of action.Method:High fat feed was used to duplicate rat model of NAFLD and small interfering RNA (SiRNA)technology was used to inhibit the expression of PGC-lo~.Sixty SD rats were randomly divided into 6groups including normol group,model group,fistular onion group,transfection group,blank transfection group,fistular onion transfection group.The structure of liver tissues was observed by using hematoxylin-eosin (HE)staining;levels of serum aspartate aminotransferase (AST),alanine aminotransferase (ALT),total cholesterol (TC),and triglyceride (TG).Real-time polymerase chain reaction (Real-time PCR) and Western blot was used to detect the mllNA and protein expression of PGC-lα,PEPCK,G6Pase.Result:The model group showed obvious hepatic steatosis,increased plasma viscosity (low shear,medium shear,high shear) and whole blood viscosity to varying degrees (P <0.05,P <0.01),significantly increased serum ALT,AST,TC and TG levels (P <0.05)and significantly decreased PGC-la,PEPCK,G6Pase mRNA and protein expression levels as compared with normal group (P <0.05).After intervention by Allii Fistulasi Bulbus extract,the fatty degeneration of the liver was significantly reduced;levels of serum ALT,AST,TC and TG were decreased (P < 0.05),and PGC-lα,PEPCK and G6Pase mRNA and protein expression levels were significantly increased (P < 0.05).In Allii Fistulasi Bulbus transfection group,even the same dose of Allii Fistulasi Bulbus extract was given, fatty degeneration of the liver was not significantly improved as compared with the model group,and no significant difference was shown in PGC-lα,PEPCK and G6Pase mRNA and protein expression levels (P <0.05). Conclusion:Allii Fistulasi Bulbus extract can improv

关 键 词：葱白提取物 非酒精性脂肪肝 肝线粒体 过氧化物酶体增殖物激活受体γ辅助激活因子-1α(PGC-1α) 磷酸烯醇式丙酮酸羧激酶(PEPCK) 葡萄糖6磷酸酶(G6Pase) 

分 类 号：R2-0[医药卫生—中医学] R22