纳米二氧化硅和铅复合暴露对斑马鱼幼鱼甲状腺内分泌系统的毒性影响  

Effect of the Lead Bioconcentration and Toxicity Combined with Silicon Dioxide Nanoparticles on the Thyroid Endocrine System of Zebrafish Larvae

作　　者：朱立一 何伟 朱璧然 ZHU Li-yi;HE Wei;ZHU Bi-ran(School of Computer Science and Information Engineering,Hubei University,Wuhan 430062,China;Institute of Hydmhiology,Chinese Academy of Sciences,Wuhan 430072,China;School of Basic Medical Sciences,Hubei University of Chinese Medicine,Wuhan 430065,China)

机构地区：[1]湖北大学计算机与信息工程学院,湖北武汉430062 [2]中国科学院水生生物研究所,湖北武汉430072 [3]湖北中医药大学基础医学院,湖北武汉430065

出　　处：《长江流域资源与环境》2018年第11期2588-2596,共9页Resources and Environment in the Yangtze Basin

基　　金：湖北省教育厅科学技术研究计划青年人才项目(Q20161011);湖北中医药大学"青苗"计划(N10040203)

摘　　要：纳米颗粒(NPs)因其广泛的应用而备受关注。NPs会改变重金属(如Pb)在生物体内的富集和毒性作用。因此,研究选择国际公认的模式动物斑马鱼(Danio rerio)为研究对象,开展纳米二氧化硅(nano-SiO_2)对Pb在鱼体内的积累和甲状腺内分泌系统干扰的研究。受精2 h后的斑马鱼胚胎,单独暴露于环境相关浓度的Pb(如0,5,10和20μg/L)或与无效应浓度nano-SiO_2(如25μg/L)复合暴露至144 h。结果表明:和nanoSiO_2复合暴露后,斑马鱼幼鱼体内Pb的含量显著高于同浓度下Pb单独暴露(P<0. 05)。此外,Pb或nanoSiO_2单独暴露都不会显著改变斑马鱼幼鱼体内甲状腺激素(T4和T3)的含量(P>0. 05);但当两者复合暴露后,斑马鱼幼鱼体内T4和T3含量显著低于对照组或同浓度下Pb单独暴露组(P<0. 01和P<0. 05)。在Pb单独或与nano-SiO_2复合暴露下,tshβ基因的表达都显著上调,但ttr和tg基因的表达都显著下调,并且复合暴露造成基因表达水平的改变较同浓度下Pb单独暴露相比更明显(P<0. 05)。上述结果指出,nano-SiO_2会增加Pb在生物体内的富集,从而对斑马鱼幼鱼的甲状腺内分泌系统造成更严重的影响。Nanoparticles (NPs)have attracted considerable attention because of their wide range of applications. Interactions between NPs and heavy metals (eg.,lead )in the aquatic environment may modify toxicant bioavailability and consequently the toxicant's environmental fate and toxicity.Therefore,we investigated the influence of silicon dioxide nanopartieles (nano-SiO2)on the bioavailability and toxicity of Pb,and also its effects on the thyroid endocrine system of zebrafish (Danio rerio)larvae.Zebrafish embryos after post-fertilization of 2hours were exposed to Pb alone (0,5,10and 20μg/L)or in combination with nano-SiO2(25μg/L)until 144hours.The results showed that the bioconcentration of Pb significantly enhanced when combined with nano- SiO2than without (P<0.05).The exposure of zebra_fish embryos/larvae to either Pb or nano-SiO2alone did not cause any significant difference in the thyroid hormone contents (T4and T3)(P>0.05),whereas the contents of both T4and T3significantly decreased under the co-exposure of Pb and nano-SiO2(P<0.01and P<0.05). The considerable up-regulation of tshfl gene were observed in Pb groups no matter with or without nano-SiO2 treatment,while the transcription of ttr and tg genes were remarkably down-regulated by Pb treatment alone,and even further down-regulated under the co-exposure (P<0.05).These results indicate that nano-SiO2enhances the bioconcentration of Pb,thus leading to thyroid endocrine disruption in zebrafish larvae.

关 键 词：铅 纳米二氧化硅 复合暴露 甲状腺干扰效应 斑马鱼(Danio rerio) 

分 类 号：R994.6[医药卫生—毒理学]