血吸虫丝氨酸/苏氨酸-蛋白激酶RIO2的生物学信息预测  

Bioinformatics Predicition of Schistosoma Japonicum Serine Threonine-protein Kinase RIO2

作　　者：赵珍[1] 阿孜尔古丽·阿布都克日木[2] 屈艳琳 戴军[3] 丁剑冰[2] 于涛[4] ZHAO Zhen;AZIERGULI Abudukerimu;QU Yanlin;DAI Jun;DING Jianbin;YU Tao(Jining First People's Hospital,Jining 272000,Shandong Province,China;Basic Medical College of Xinjiang Medical University,Wulumuqi 830011,Xinjiang Uygur Autonomous Region,China;Jining Medical University,Jining 272000,Shandong Province,China;Shandong Institute of Parasitical Disease,Jining 272033,Shandong Province,China)

机构地区：[1]山东省济宁市第一人民医院,山东济宁272000 [2]新疆医科大学基础医学院,乌鲁木齐830011 [3]济宁医学院,山东济宁272000 [4]山东省寄生虫病防治研究所,山东济宁272033

出　　处：《寄生虫病与感染性疾病》2018年第4期182-185,191,共5页Parasitoses and Infectious Diseases

基　　金：山东省自然科学基金项目(项目编号:ZR2013HL014);济宁市科技局立项课题(项目编号:2009-56-34)

摘　　要：目的应用在线软件分析血吸虫丝氨酸/苏氨酸-蛋白激酶RIO2抗原蛋白的生物学信息,为研制新型血吸虫病基因疫苗提供理论基础。方法采用蛋白质分析系统Protparam(http:∥expasy.org/tools/protparam.html)分析血吸虫丝氨酸/苏氨酸-蛋白激酶RIO2蛋白的理化性质;利用软件Protscale(http:∥www.espasy.org/cgibin/protscale.p1)蛋白质在线分析RIO2蛋白的亲(疏)水性;在线软件预测血吸虫RIO2丝氨酸/苏氨酸-蛋白激酶蛋白的二级结构,利用PyMOLviewer软件作图构建三级结构模型。结果利用在线软件预测血吸虫RIO2抗原蛋白的二级结构中α螺旋结构占41.01%,β-折叠占11.63%,无规则卷曲占34.25%。负电荷残基(Asp+Glu)数为55,正电荷残基(Arg+Lys)数为77,不稳定系数为50.41,预测为不稳定蛋白;总平均亲水性系数为-0.633,可能为亲水性蛋白质;预测RIO2蛋白糖基化位点在31、96、176、260、330和369氨基酸处,且存在在1个丝氨酸、1个苏氨酸、2个酪氨酸磷酸化。结论生物信息学分析血吸虫RIO2含有磷酸化位点和糖基化位点。因此推测其可能具有免疫原性,可为血吸虫高效表位疫苗的研发提供参考,为血吸虫病的临床诊断与治疗提供新的靶标。Objective The online software was used to analyze the biological information of the schistosomiasis Japonicum serine threonine-protein kinase RIO2 antigen protein, which provided a theoretical basis for the development of a new schistosomiasis gene vaccine.Methods The paper analyzed the physicochemical properties of RIO2 protein by using Protparam ( http: // expasy.org /tools/protparam.html).The On-line analysis of the affinity ( R) water of RIO2 protein was conducted by using the software Protscale ( http: //www. espasy.org /cgibin /protscale.p1).The online software was used to predict the secondary structure of the Schistosoma japonicum RIO2 serine /threonine-protein kinase proteinand the Py MOL viewer software was used to construct a three -level structural model. Results The online prediction software was used to predict the secondary structure of the Schistosoma japonicum /threonine - protein kinase RIO2,the results showed that the antigen protein accounted for 41.01%,β-folded accounted for 11. 63%,and random curl accounted for 34. 25%. The number of negatively charged residues ( Asp+Glu) was 55,the number of positively charged residues ( Arg+Lys) was 77,and the protein instability coefficient was 50. 41.The protein was predicted to be an instable protein with a total average hydrophilicity of-0. 633, which meant it would be a hydrophilic protein.A glycosylation site was presented at the 31,96,176,260,330,369 amino acid positions of the RIO2 protein,and a prediction site for phosphorylation at 1 serine,1 threonine,and 2 tyrosine,Conclusion Bioinformatics analysis of Schistosoma japonicum RIO2 contains phosphorylation sites and glycosylation sites,which implicates that it may be immunogenic.This finding can provide references for the research of schistosomiasis serine /threonine - protein kinase RIO2 antigenicity research and high-efficiency epitope vaccine,and provide new targets for clinical immunodiagnosis and drug therapy of schistosomiasis.

关 键 词：日本血吸虫 RIO2蛋白 抗原表位 生物信息学 

分 类 号：R383.24[医药卫生—医学寄生虫学]