体外循环诱发大鼠肠屏障损伤的内源性保护机制:与肠神经胶质细胞的关系  

Endogenous protective mechanism of intestinal barrier injury induced by cardiopulmonary bypass in rats:the relationship with enteric glia cells

作　　者：伊小婷[1] 常畅 孙莹杰[1] Yi Xiaoting;Chang Chang;Sun Yingjie(Department of Anesthesiology,General Hospital of Shenyang Military Region,Shenyang 210016,China)

机构地区：[1]沈阳军区总医院麻醉科,110015

出　　处：《中华麻醉学杂志》2018年第8期921-924,共4页Chinese Journal of Anesthesiology

摘　　要：目的 评价体外循环(CPB)诱发大鼠肠屏障损伤的内源性保护机制与肠神经胶质细胞的关系.方法 清洁级成年雄性SD大鼠48只,体重400~500 g,采用随机数字表法将其分为2组(n=24):假手术组(S组)和CPB组.于CPB开始时(T0)、CPB 60 min(T1)、CPB结束后2 h(T2)和CPB结束后6 h(T3)时处死大鼠,取肠组织,HE染色观察肠组织病理学结果;Western blot法检测肠黏膜紧密连接蛋白ZO-1、occludin及胶质纤维酸性蛋白(GFAP)、钙结合蛋白(S-100)的表达水平;免疫组化法测定GFAP阳性表达情况.结果 与S组比较,CPB组T1-3时肠组织GFAP和S-100表达上调,occludin和ZO-1表达下调(P<0.05),GFAP阳性表达增强,肠黏膜损伤显著.结论 肠神经胶质细胞活化水平增强可能参与了CPB诱发大鼠肠屏障损伤的内源性保护机制.Objective To evaluate the relationship between endogenous protective mechanism of intestinal barrier injury induced by cardiopulmonary bypass ( CPB) and enteric glia cells in rats. Methods Forty-eight clean-grade adult male Sprague-Dawley rats, weighing 400-500 g, were divided into 2 groups (n=24 each) using a random number table method: sham operation group (group S) and CPB group. Rats were sacrificed at the beginning of CPB, 60 min of CPB, and 2 and 6 h after CPB, and the intestinal tissues were removed for examination of pathological changes ( by HE staining) and for determina-tion of the expression of ZO-1, occludin, glial fibrillary acidic protein ( GFAP ) and calcium-binding pro-tein ( S-100) and the positive expression of GFAP ( by immunohistochemical method) . Results Compared with group S, the expression of GFAP and S-100 was significantly up-regulated at 60 min of CPB and 2 and 6 h after CPB, the expression of ZO-1 and occludin was down-regulated (P<0. 05), the positive expres-sion of GFAP was enhanced, and the intestinal mucosal injury was marked in group CPB. Conclusion The enhanced activation of enteric glia cells may be involved in the endogenous protective mechanism of in-testinal barrier injury induced by CPB in rats.

关 键 词：心肺转流术 肠黏膜 神经胶质 

分 类 号：R614[医药卫生—麻醉学]