鞘内注射右美托咪定对内脏痛大鼠脊髓背角P物质和c-fos表达的影响  

Effect of intrathecal dexmedetomidine on expression of substance P and c-fos in spinal dorsal horns of rats with visceral pain

作　　者：张广儒 冷玉芳[1] 吕兴华[1] 彭晨媚 顾凤香 吕继鹏 Zhang Guangru;Leng Yufang;Lyu Xinghua;Peng Chenmei;Gu Fengxiang;Lyu Jipeng(Department of Anesthesiology,First Hospital of Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州大学第一医院麻醉科,730000

出　　处：《中华麻醉学杂志》2018年第8期960-963,共4页Chinese Journal of Anesthesiology

摘　　要：目的 评价鞘内注射右美托咪定对内脏痛大鼠脊髓背角P物质(SP)和c-fos表达的影响.方法 清洁级健康成年雄性SD大鼠32只,体重250~300 g,采用随机数字表法分为4组(n=8):对照组(C组)、内脏痛组(VP组)、右美托咪定组(D组)和右美托咪定+阿替美唑组(DA组).VP组、D组和DA组腹腔注射0.9%乙酸10 ml∕kg制备大鼠内脏痛模型,C组腹腔注射等容量生理盐水.于模型制备前10 min,D组和DA组分别鞘内注射右美托咪定20μl(1μg∕kg),右美托咪定1μg∕kg+阿替美唑1μg∕kg,共20μl,C组和VP组鞘内注射生理盐水20μl.于腹腔注射后1 h时记录内脏痛指数,随后处死大鼠取脊髓L4~6节段,采用免疫组化法检测SP和c-fos表达.结果 与C组比较,VP组、D组、DA组内脏痛指数升高,脊髓背角SP和c-fos表达上调(P<0.05);与VP组比较,D组和DA组内脏痛指数降低,脊髓背角SP和c-fos表达下调(P<0.05);与D组比较,DA组内脏痛指数升高,脊髓背角SP和c-fos表达上调(P<0.05).结论 鞘内注射右美托咪定可通过抑制脊髓背角SP和c-fos表达减轻大鼠内脏痛,这种作用与脊髓背角α2肾上腺素能受体有关.Objective To evaluate the effect of intrathecal dexmedetomidine on expression of sub-stance P (SP) and c-fos in the spinal dorsal horns of rats with visceral pain. Methods Thirty-two clean-grade healthy adult male Sprague-Dawley rats, weighing 250-300 g, were divided into 4 groups ( n=8 each) using a random number table method: control group (C group), visceral pain group (VP group), dexmedetomidine group (D group) and dexmedetomidine plus atipamezole group (DA group). VP, D and DA groups received intraperitoneal injection of 0. 9% acetic acid 10 ml∕kg to establish the model of visceral pain, while group C received the equal volume of normal saline instead. At 10 min before the model was es-tablished, dexmedetomidine 20 μl (1μg∕kg) and dexmedetomidine 1μg∕kg plus atipamezole 1μg∕kg (20μl) were intrathecally injected in D and DA groups, respectively, and the equal volume of normal saline 20μl was given instead in C and VP groups. Visceral pain index ( VPI) was recorded at 1 h after intraperito-neal injection, and then rats were sacrificed, and the dorsal horns of the spinal cord ( L4-6 ) were removed for determination of the expression of SP and c-fos by immunohistochemistry. Results Compared with group C, VPI was significantly increased, and the expression of SP and c-fos was up-regulated in VP, D and DA groups (P<0. 05). Compared with group VP, VPI was significantly decreased, and the expression of SP and c-fos was down-regulated in D and DA groups (P<0. 05). Compared with group D, VPI was signifi-cantly increased, and the expression of SP and c-fos was up-regulated in group DA ( P<0. 05) . Conclusion Intrathecal dexmedetomidine reduces the visceral pain through inhibiting the expression of SP and c-fos in&nbsp;the spinal dorsal horns, which is related to the α2-adrenergic receptor in spinal dorsal horns of rats.

关 键 词：右美托咪啶 注射 脊髓 内脏 疼痛 P物质 原癌基因蛋白质C-FOS 

分 类 号：R614[医药卫生—麻醉学]