水稻簇生穗基因CPB4的精细定位  

Fine-Mapping CPB4 Gene Associated with Clustered Spikelets in Rice

作　　者：姜玲 姜宁[3] 曹黎明 董世青[1,2] 杨金水 陆平利[1,2] 罗小金 JIANG Ling;JIANG Ning;OAO Liming;DONG Shiqing;YANG Jinshui;LU Pingli;LUO Xiaojin(Institute of Genetics,School of Life Sciences,Fudan University,Shanghai 200438,China;State Key Laboratory of Genetic Engineering,Fudan University,Shanghai 200438,China;State Key Laboratory of Genetic Engineering,Department of Biostatistics,School of Life Sciences,Fudan University,Shanghai 200438,China;Crop Breeding And Cultivation Research Institute,Shanghai Academy of Agricultural Science,Shanghai 201403,China)

机构地区：[1]复旦大学生命科学学院遗传学研究所,上海200438 [2]复旦大学遗传工程国家重点实验室,上海200438 [3]复旦大学生命科学学院生物统计系,上海200438 [4]上海市农业科学院作物育种栽培研究所,上海201403

出　　处：《复旦学报（自然科学版）》2018年第6期757-766,共10页Journal of Fudan University：Natural Science

基　　金：国家自然科学基金面上项目(31471461);上海市科技兴农重点攻关项目(沪农科攻字(2014)第7-1-2号)

摘　　要：本研究鉴定了一个穗型突变体T637,表现为簇生穗型,穗粒数减少,结实率降低,籽粒变小,千粒重下降.遗传分析发现穗型突变由隐性单基因控制;通过基因芯片检测和SSR标记分析最终将目标基因定位在第4号染色体标记RM2439和RMZL74之间1.3 Mb范围内.随后通过全基因组重测序,发现突变体中LOC_Os04g39430基因第4外显子上有一段4 262 bp序列插入,因此确定其为候选基因,并命名为CPB4(CLUSTERED PRIMARY BRANCH 4).通过分析亚洲栽培稻特青(O.sativa L.)和T637对不同浓度24-epiBL(24-表油菜素内酯)的敏感程度,确认T637为油菜素内酯敏感型突变体.qRT-PCR分析表明,突变体T637中BR生物合成相关基因D2和BRD2以及BR信号转导相关基因BRI1,OsBAK1,OsLIC1,TUD1,BU1在幼穗中的表达量均显著降低.A panicle nature mutant T637 was obtained which shows clustered primary branch of panicle and smaller seed,the grain number of main panicle,seed setting rate and the 1000-grain weight of T637 was decreased apparently compared with the wild-type plant.To begin with,the T637 mutant was proved to be controlled by a single recessive nuclear gene via genetic analysis.Then the candidate gene,named CPB4,was finally mapped to a 1.3Mb range between RM2439 and RMZL74 by applying the gene chip technology and several simple sequence repeat (SSR)markets to the recessive and dominant plants in the F2population.Furthermore,a 4262bp sequence insertion was identified in exon 4 of the candidate gene LOC_ Os04g39430 in T637 mutant by the Bulked-Segregant Analysis (BSA).Finally,lamina inclination assays between Teqing and T637 indicated that T637 was a BR-related mutant and was sensitive to 24-epiBL treatment.Quantitative RT-PCR (qRT-PCR)analysis showed that the expression levels of some BR biosynthesis genes (D2,BRD2)and some BR-signalling genes (BRI1,OsBAK1, OsLIC1,TUD1,BU1) were significantly decreased in T637 compared to the Teqing.

关 键 词：水稻 簇生穗 精细定位 CPB4 油菜素内酯 

分 类 号：Q943.2[生物学—植物学]