共振光散射光谱法测定牛血清蛋白  

Determination of Bovine Serum Albumin by Resonance Light Scattering Spectroscopy

作　　者：尚永辉[1,2] 刘静[1,2] 刘建波[1,2] 郭颖[1,2] 陈燕[1,2] 张萍 SHANG Yonghui;LIU Jing;LIU Jianbo;GUO Ying;CHEN Yan;ZHANG Ping(School of Chemistry and Chemical Engineering,Xianyang Normal University,Xianyang 712000,China;Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry,Institute of Analytical Science, Northwest University,Xi'an 710069,China)

机构地区：[1]咸阳师范学院化学与化工学院,咸阳712000 [2]西北大学分析科学研究所陕西省电分析化学重点试验室,西安710069

出　　处：《理化检验（化学分册）》2018年第12期1441-1444,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金项目(21475113);陕西省软科学研究计划项目(2015KRM164);咸阳师范学院‘青蓝人才’项目(XSYQL201507);咸阳师范学院国家级大学生创新创业训练计划项目(201810722006)

摘　　要：于10mL比色管中依次加入pH 3.29的Britton-Robinson缓冲溶液3.00mL,一定体积的8.8×10-6 mol·L^(-1)牛血清蛋白溶液,1.00×10-3 mol·L^(-1)的2-[2-(5,6-二氯苯并噻唑)偶氮]-5-二甲氨基苯甲酸溶液0.40mL,再加入35g NaCl,以水定容,摇匀,在35℃反应3h后,在激发波长和发射波长均为524nm处测定体系的共振光散射强度。结果发现,牛血清蛋白的浓度在1.00～25.00μmol·L^(-1)内与体系的共振光散射强度呈线性关系,检出限(3S/N)为0.33μmol·L^(-1)。方法用于测定牛尿中牛血清蛋白的含量,加标回收率在99.6%～102%之间,测定值的相对标准偏差(n=6)在1.8%～5.8%之间。3.00 mL of Britton-Robinson buffer solution(pH 3.29),certern volume of 8.8×10-6 mg·L^-1bovine serum albumin solution,and 0.40 mL of 1.00×10-3 mg·L^-1 2-[2-(5,6-dichlorobenzothiazolyl)azo]-5-dimethylaminobenzoic acid solution,were added into a 10 mL colorimetric tube successively.After 35 g of NaCl was added,the mixture was made up to 10 mL with water,shaked well,and reacted at 35 ℃ for 3h.Then the resonance light scattering intensity of the system was mesured at the excitation wavelength and the emission wavelength of 524 nm.Linear relationship was found between the resonance light scattering intensity of the system with the concentration of bovine serum albumin in the range of 1.00-25.00μmg·L^-1,and the detection limit(3S/N)was 0.33μmg·L^-1.The method was applied to determination of the content of bovine serum albumin in bovine urine,gving values of recovery between 99.6% and 102%,and RSDs(n=6)between 1.8%-5.8%.

关 键 词：共振光散射光谱法 牛血清白蛋白 2-[2-(5 6-二氯苯并噻唑)偶氮]-5-二甲氨基苯甲酸 

分 类 号：O657.3[理学—分析化学]