酸化甘油溶血试验在红细胞储存研究中的应用  

Application analysis of the acidified glycerol hemolysis test in red cell storage

作　　者：王子[1,2] 查占山 李津婴[1] 郭菲[1] 韦燕萍[1] 唐河山[1] 钱宝华[1] WANG Zi;CHA Zhanshan;LI Jiny-ing;GUO Fei;WEI Yanping;TANG Heshan;QIAN Baohua(Department of Transfusion,Changhai Hospital,The Sec-ond Military Medical University,Shanghai 200433,China;Department of Transfusion,Kunming General Hospital of PLA)

机构地区：[1]第二军医大学长海医院输血科,上海200433 [2]解放军昆明总医院输血科

出　　处：《中国输血杂志》2018年第10期1142-1144,共3页Chinese Journal of Blood Transfusion

摘　　要：目的探索酸化甘油溶血试验在红细胞储存损伤研究中应用的可能性。方法随机采集无偿献血者全血标本20份,4℃储存5d。分别于储存d1,d3和d5采用AGLT和EOFT来检测渗透脆性。AGLT计算50%未溶血率对应的时间,EOFT计算50%溶血率对应的NaCl浓度。比较2种试验方法在检测红细胞渗透脆性上的差异。结果AGLT50%未溶血率所对应的时间从(34.60±1.40)s缩短到(28.71+±2.50)s(P<0.05),EOFT50%溶血率所对应的NaCl浓度从(4.36±0.21)g/L增加到(4.89±0.26)g/L(P<0.05);AGLT在性别之间无显著差异(P>0.05),EOFT在性别之间也无显著差异(P>0.05)。结论AGLT计算结果精确,物料耗费少,操作耗时短,是一种可以用于红细胞储存损伤研究的检测方法。Objective To explore the possibility of applying the acidified glycerol hemolysis test in the lesion study of red blood cells during storage. Methods Samples of 20 blood donors were randomly collected and stored at 4 ℃ for 5 days.Samples were tested on 1,3,5 days respectively by AGLT and EOFT. Time of the 50% non-hemolysis rate were calculated in AGLT and Na Cl concentration of 50% hemolysis rate were calculated in EOFT. Comparison of the differences between the two methods in detecting osmotic fragility of red blood cells was made. Results In AGLT,the time of 50% non-hemolysis rate shortened with the extension of storage time( P<0. 05). In EOFT,the Na Cl of 50% hemolysis rate increased with extended storage time( P<0. 05). AGLT showed no significant difference between gender( P> 0. 05),EOFT also showed no significant difference between gender( P>0. 05). Conclusion AGLT is an accurate,resource and time saving method for researching red blood cell storage lesion.

关 键 词：红细胞 储存损伤 酸化甘油溶血试验 渗透脆性 

分 类 号：R457.1[医药卫生—治疗学] R331.141[医药卫生—临床医学]