梅毒螺旋体抗体单试剂反应性献血者归队策略研究  

Reentry strategy for single-reactive blood donors in anti-TP ELISA tests

作　　者：王东[1] 臧亮[1] 陈辉[1] 邓雪莲[1] 梁晓华[1] WANG Dong;ZANG Liang;CHEN Hui;DENG Xuelian;LIANG Xiaohua(Liaoning Dalian Blood Center,Dalian 116001,China.)

机构地区：[1]大连市血液中心

出　　处：《中国输血杂志》2018年第10期1177-1180,共4页Chinese Journal of Blood Transfusion

基　　金：卫生部医药卫生科技发展研究中心专项课题(卫计中[2012]12号28-8-3)

摘　　要：目的通过跟踪复查,摸索抗-TP单试剂反应性献血者的归队策略。方法对抗-TP单试剂反应性献血者进行多次跟踪复查,包括酶联免疫法(ELISA)和梅毒螺旋体抗体明胶颗粒凝集试验(TPPA),每次时间间隔≥3个月。献血者的复查判定:3种抗-TP检测试剂中≥2种呈反应性,献血者继续屏蔽,不再参与跟踪;其余的情况,献血者继续跟踪观察。结果2013年1月1日-2015年9月30日间,在415名抗-TP单试剂反应性献血者中有65人(15.66%)参与了研究,跟踪时间跨度95-892d(中位数371d)。全部跟踪检测的TPPA结果为无反应性,ELISA再次反应性的献血者有20人(30.77%):参与1次复查41人,再次反应性14人(34.15%);参与2次14人,再次反应性5人(35.71%);参与3次10人,再次反应性1人(10.00%);共有4人发生了跟踪结果反应性与无反应性交替出现的情况。结论抗-TP单试剂反应性献血者归队策略为:跟踪复查≥2次,每次时间间隔≥3个月,若末次结果中任意试剂出现反应性则继续屏蔽;若末次结果无反应性即可恢复献血资格。Objective To propose an viable reentry strategy for single-reactive blood donors in Anti-TP ELISA tests.Methods Follow-up samples from research donors were collected according to a schedule with a 3-month interval. Reconfirmation tests were then performed on the collected samples using routine and supplementary tests( Enzyme-linked immunosorbent assay,ELISA; Treponema pallidum particle agglutination assay,TPPA). Should more than 1 reactive result appeared in the 3 tests( 2 ELISA kits and 1 TPPA kit),the donor is determined unqualified while other result cases require further follow-up. Results During January 1 st 2013 to September 30 th 2015,sixty-five of 415 blood donors were single-reactive in the Anti-TP ELISA tests and were therefore enrolled in this study. The follow-up periods ranged from 95 d-892 d( median 371 d). All follow-up samples’ TPPA results were non-reactive while reactive samples were detected in 20 of the 65( 30. 77%)donors. 41 of the qualified donors went through the follow-up retesting once,14 twice and 10 three-time. 14,5 and 1 samples were detected as reactive respectively. Conclusion The reentry of these single-reactive patients requires at least twice or more follow-up tests with an interval of more than 3 months. Any reactive results leads to further follow-ups. Only the all-clear donors could be assigned to reenter blood donation.

关 键 词：梅毒螺旋体抗体 献血者归队 血清学检测 

分 类 号：R457[医药卫生—治疗学] R446[医药卫生—临床医学]