球孢白僵菌不同碳氮源的研究及对烟粉虱的时间-剂量-死亡率模型分析  

Effects of different carbon and nitrogen sources on Beauveria bassiana and a time-dose-mortality model of effectiveness of B. bassiana as a biological control for Bemisia tabaci

作　　者：姜灵[1] 谢婷 洪波[1] 王新谱[1] 贾彦霞[1] JIANG Ling;XIE Ting;HONG Bo;WANG Xin-Pu;JIA Yan-Xia(School of Agriculture,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学农学院,银川750021

出　　处：《应用昆虫学报》2018年第5期912-918,共7页Chinese Journal of Applied Entomology

基　　金：宁夏"十三五"重大科技专项(2016BZ09-03)

摘　　要：【目的】明确不同碳、氮源对球孢白僵菌Beauveria bassiana生长的影响,确定适合该菌株生长的碳、氮源;通过研究球孢白僵菌对烟粉虱Bemisia tabaci的致病力,评价球孢白僵菌防治烟粉虱的潜力。【方法】在培养基中添加不同碳、氮源,研究其对球孢白僵菌生长速率和产孢量的影响,用浸叶法研究不同孢子浓度(1×10~4、1×10~5、1×10~6、1×10~7、1×10~8个/mL)的球孢白僵菌对烟粉虱的致病力并利用时间-剂量-死亡率模型对数据进行拟合,分析该模型的时间效应和剂量效应。【结果】蔗糖作为碳源、酵母作为氮源时球孢白僵菌的生长速率快和产孢量高;利用时间-剂量-死亡率模型对测定的数据进行拟合分析得出,随着接种天数的增加,球孢白僵菌的LC_(50)、LC_(90)逐渐降低,剂量效应增强;随着孢子浓度的增加,LT50逐渐降低,时间效应增强。【结论】球孢白僵菌的合适碳源为蔗糖,最适氮源为酵母。该菌株对烟粉虱有较强致病力,1×10~8个/mL的孢子浓度可作为防治烟粉虱的理想浓度,本试验为球孢白僵菌对烟粉虱的生物防治提供有力依据。[Objectives] To understand the effects of different carbon and nitrogen sources on the growth of Beauveria bassiana, identify optimum carbon and nitrogen sources for this species, investigate the virulence of Bemisia tabaci and evaluate the potential of B. bassiana as a biological control for this pest.【Methods】 The growth and sporulation of B. bassiana were investigated using growth medium with different carbon and nitrogen sources, and the virulence of B. bassiana to B. tabaci was measured by immersing leaves in a conidial suspension at concentrations of 1×104,1×105,1×106,1×107,1×108 conidia/mL. A time-dose-mortality model was used to analyze the effects of dosage and time on the virulence of B. bassiana to B. tabaci.【Results】 B. bassiana maintained higher growth and sporulation rates in medium containing sucrose and yeast. A time-dose-mortality model showed that LC50 and LC90 decreased over time, whereas the dose effect increased. The LT50 decreased at higher conidial suspension concentrations and the time effect increased. 【Conclusion】 The best carbon source was sucrose and the best nitrogen source was yeast. B. bassiana had high virulence to B. tabaci and the best control was achieved at a concentration was 1×108 conidia/mL. These results indicate that B. bassiana is a promising biological control agent for B. tabaci.

关 键 词：球孢白僵菌 碳源 氮源 致病力 时间-剂量-死亡率模型 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]