贵州党参的HPLC特征图谱研究  

Study on the HPLC characteristic chromatogram of Codonopsis tubulosa

作　　者：柏彩红 王波[1] 徐文芬[1] 石玲玲[1] 孙庆文[1] BAI Caihong;WANG Bo;XU Wenfen;SHI Lingling;SUN Qingwen(Guiyang University Traditional Chinese Medicine,Guiyang,Guizhou,550025P.R.China)

机构地区：[1]贵阳中医学院

出　　处：《华西药学杂志》2018年第6期628-634,共7页West China Journal of Pharmaceutical Sciences

基　　金：贵州省教育厅一流学科建设项目(GNYL[2017]008);贵州省普通高等院校工程研究中心项目(黔教合KY字[2017]018);贵州省科学技术基金项目(黔科合J字[2013]2061号);贵州省中药、民族药饮片标准研究课题(DBYP52523)

摘　　要：目的研究贵州党参的HPLC特征图谱,为研究其物质基础提供参考。方法采用HPLC法,以Agilent Zorbax SB-C18(150mm×4.6mm,5μm)为色谱柱,乙腈-0.4%磷酸水溶液为流动相进行梯度洗脱,流速1.0mL·min^-1,检测波长270nm,柱温30℃,进样量10μL;运用《中药色谱指纹图谱相似度评价系统2004A、B版》和SPSS 18.0软件对17批贵州党参药材进行相似度分析和主成分分析。结果建立了贵州党参药材的特征图谱,共标定15个特征峰,并指认了1个特征峰党参炔苷。相似度评价结果表明:17批贵州党参药材的相似度为0.616~0.977。主成分分析结果表明:17批贵州党参药材中12批样品较集中,5批样品较分散。结论所用方法稳定可靠,较全面地反映了贵州党参药材中的化学成分信息,可为其药材的质量控制提供依据。OBJECTIVE To study the HPLC characteristic chromatogram of Codonopsis tubulosa and to provide the reference for the research of C. tubulosa. METHODS HPLC method was carried out on Agilent Zorbax SB-C18( 150 mm × 4. 6 mm,5 microns) with0. 4% acetonitrile-phosphate solution as mobile phase in gradient elution at a column temperature of 30 ℃. The detection wavelength was 230 nm. The flow rate was 1. 0 m L·min–1 and the injection volume was 10 μL. The similarity analysis and principal component analysis of 17 batches of C. tubulosa were performed by using the Chinese medicine chromatographic fingerprint similarity evaluation system 2004 A,B and SPSS18. 0 software. RESULTS The HPLC characteristic chromatogram of C. tubulosa was established. 15 common peaks were specified,and one of them was identified as lobetyolin. The results of similarity evaluation showed the similarity of17 batches of C. tubulosa was 0. 616-0. 977. The results of principal component analysis showed that 12 batches of C. tubulosa were more concentrated and 5 batches of C. tubulosa were more dispersed. CONCLUSION The method is stable and reliable,which can comprehensively reflect the chemical composition information of C. tubulosa and provide the scientific basis for the quality control of C.tubulosa.

关 键 词：贵州党参 高效液相色谱法 特征图谱 党参炔苷 特征峰 相似度分析 主成分分析 质量控制 

分 类 号：R917[医药卫生—药物分析学]