Detection of EBV and HHV6 in the Brain Tissue of Patients with Rasmussen's Encephalitis  

作　　者：Dong Liu Xin Wang Yisong Wang Peigang Wang Dongying Fan Sichang Chen Yuguang Guan Tianfu Li Jing An Guoming Luan 

机构地区：[1]department of neurosurgery,sanbo brain hospital,capital medical university,Beijing 100093,China [2]department of microbiology,school of basic medical science,capital medical university,Beijing 100069,China [3]department of neurosurgery,xuanwu hospital of capital medical university,Beijing 100053,China [4]beijing key laboratory of epilepsy [5]center of epilepsy,beijing institute for brain disorders,Beijing 100093,China

出　　处：《Virologica Sinica》2018年第5期402-409,共8页中国病毒学（英文版）

基　　金：supported by the National Natural Science Foundation of China(81701992,81471957,and 81671971);the Beijing Municipal Natural Science Foundation(7144217);the Capital Applied Clinic Research Programs of Science and Technology(Z131107002213171);the Beijing Rising-star Plan of Science and Technology(Z141107001814042);Scientific Research Common Program of Beijing Municipal Commission of Education(KM201610025001)

摘　　要：Rasmussen's encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis,intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8?T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8?T cells are involved in the pathogenesis of RE.Rasmussen's encephalitis (RE) is a rare pediatric neurological disorder, and the exact etiology is not clear. Viral infection may be involved in the pathogenesis of RE, but conflicting results have reported. In this study, we evaluated the expression of both Epstein-Barr virus (EBV) and human herpes virus (HHV) 6 antigens in brain sections from 30 patients with RE and16 control individuals by immunohistochemistry. In the RE group, EBV and HHV6 antigens were detected in 56.7% (17/30) and 50% (15/30) of individuals, respectively. In contrast, no detectable EBV and HHV6 antigen expression was found in brain tissues of the control group. The co-expression of EBV and HHV6 was detected in 20.0% (6/30) of individuals. In particular, a 4-year-old boy had a typical clinical course, including a medical history of viral encephalitis,intractable epilepsy, and hemispheric atrophy. The co-expression of EBV and HHV6 was detected in neurons and astrocytes in the brain tissue, accompanied by a high frequency of CD8^+T cells. Our results suggest that EBV and HHV6 infection and the activation of CD8^+T cells are involved in the pathogenesis of RE.

关 键 词：Rasmussen’s ENCEPHALITIS (RE) Epstein-Barr VIRUS (EBV) Human HERPES VIRUS 6 (HHV6) Seizures CD8^+T cells 

分 类 号：Q[生物学]