黄虎方对HBV转基因小鼠病毒复制及TKB1-IRF7-IFNα/β通路的影响  

Influence on virus replication and TKB1-IRF7-IFNα/β pathway in HBV transgenic mice treated with HuangHu Fang

作　　者：廖婷婷[1,2] 黄群[1,2] 张传涛 辜海英[1] 杨沁[3] Liao Ting-ting;Huang Qun;Zhang Chuan-tao;Gu Hai-ying;Yang Qin(Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610075;Chengdu University of Traditional Chinese Medicine,Chengdu 610072;Leshan Traditional Chinese Medicine Hospital,Leshan 614000)

机构地区：[1]成都中医药大学附属医院,成都610075 [2]成都中医药大学,成都610072 [3]乐山市中医医院,乐山614000

出　　处：《中国抗生素杂志》2018年第12期1574-1577,共4页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(No.81102720);四川省中医药管理局中医药科技研究专项(No.2016C013);四川省中医药管理局中医药科技研究专项(No.2016Q018);四川省教育厅科研项目(No.17ZB0145)

摘　　要：目的观察黄虎方(HH方)治疗HBV转基因小鼠抗乙肝病毒(hepatitis B virus,HBV)疗效及其作用机制。方法 HBV转基因小鼠随机分为4组,苦参素组小鼠给予含150mg/(kg·d)苦参素混悬液,高、低剂量HH方组小鼠分别给予含7g/(kg·d)、2g/(kg·d) HH混悬液,均为每日1次灌胃,共5周,Elisa法检测血清及肝组织中乙型肝炎病毒表面抗原(hepatitis B virus surface antigen, HBsAg),Elisa法检测血清干扰素α(interferon-α,IFNα)、干扰素β(interferon-β,IFNβ),定量PCR法检测血清中乙型肝炎病毒脱氧核糖核酸(hepatitis B virus DNA, HBVDNA)及肝组织中HBVDNA、TANK结合激酶1(TANK-binding kinase 1, TKB1)mRNA、干扰素调控因子7(interferon regulatory factor 7, IRF7) mRNA表达。Western Bloting检测肝组织中TKB1、IRF7蛋白表达。结果高剂量HH方(7g/(kg·d))可以显著降低HBV转基因小鼠肝脏和血清中的HBsAg、HBVDNA水平,差异具有统计学意义(P<0.01, P<0.05),与苦参素组比较差异无统计学意义(P>0.05)。高剂量HH方显著增强小鼠TKB1 mRNA、IRF7 mRNA及蛋白表达,差异具有统计学意义(P<0.01,P<0.05)。高剂量HH方可显著升高血清IFNα、IFNβ,差异有统计学意义(P<0.01,P<0.05)。结论 HH方具有一定抗HBV作用,推测可能与影响TKB1、IRF7的mRNA和蛋白表达进而促进IFNα/β分泌有关。Objective To observe the anti-HBV effect and the action mechanism of HuangHu Fang (HH Fang) in HBV transgenic mice.Methods HBV transgenic mice were randomly divided into four groups.The Matrine group was fed with the suspension containing 150mg/(kg·d)Matrine,the high and low dose HH Fang groups were given the suspension containing 7g/(kg·d)and 2g/(kg·d)HH Fang,once daily for five weeks.Elisa was used to detect HBsAg in the serum and liver tissue.Elisa was also used to detect serum Interferon-α and Interferon-β.Quantitative PCR was used to detect HBVDNA in serum and HBVDNA,TANK-binding kinase 1 mRNA,and interferon regulatory factor mRNA in liver tissue.The Western Blotting method was used to detect protein expression of TKB1 and IRF7 in liver tissue.Results High dose HIt Fang (7g/(kg·d))could significantly reduce HBsAg,HBVDNA in the serum and liver of HBV transgenic mice.The difference was statistically significant (P<0.01,P<0.05)compared with the Matrine group,where the difference was not statistically significant (P>0.05).High doses HH Fang significantly increased mRNA and protein expression of TKB1 and IRF7,and the difference was statistically significant (P<0.01,P<0.05).High-dose HH Fang could significantly increase the level of IFNα and IFNβ in the serum,and the difference was statistically significant (P<0.01,P<0.05).Conclusion HH Fang had some anti-HBV effects,presumably related to influencing mRNA and protein expression of TKB1 and IRF7 to promote IFNα/β secretion.

关 键 词：黄虎方 抗乙肝病毒 作用机制 

分 类 号：R9[医药卫生—药学]