电针足三里穴对FGIDs大鼠胃肠微循环及5-HT影响研究  

Study on the Effect of the Gastrointestinal Microcirculation and 5-HT by Electroacupuncture at FGIDs Rats

作　　者：刘月露 谭文胜 朱琪 李春雨[1] 王晓燕[1] LIU Yuelu;TAN Wensheng;ZHU Qi;LI Chunyu;WANG Xiaoyan(Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China)

机构地区：[1]山东中医药大学

出　　处：《辽宁中医药大学学报》2018年第12期79-81,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然科学基金青年基金项目(81403326);山东省优秀中青年科学家科研奖励基金资助项目(2012BSB14020);山东省中医药科技发展计划项目(2011-004)

摘　　要：目的:观察电针足三里穴对功能性胃肠病(FGIDs)模型大鼠胃、肠表面微循环以及五羟色胺(5-HT)含量的影响,并探讨其作用机制。方法:综合方法复制FGIDs大鼠模型。电针足三里穴,并以阴谷穴和非经非穴为对照,每天1次,共针刺7d,激光多普勒血流仪同步监测胃、肠表面微循环血流量,采用酶联免疫法检测血:下丘脑、脊髓、胃、肠中5-HT含量的变化。结果:电针足三里穴可以明显改善胃、肠表面微循环,增加其微循环血流量(P<0.05),并可以明显降低FGIDs模型大鼠异常升高的血、下丘脑、脊髓及胃肠等各部位5-HT含量(P<0.05),且与阴谷穴、非经非穴相比,胃肠表面微循环血流量和各部位5-HT含量的变化皆有显著性差异(P<0.05)。结论:电针足三里穴可以改善FGIDs模型大鼠胃肠微循环状况而改善其症状,可能机制与整体调节各水平部位的5-HT含量有关,而且具有穴位特异性。Objective:To observe the effect of electroacupuncture(EA)at Zusanli(ST36)on microcirculation of gastric and intestinal surface and the content of 5-HT in the functional gastrointestinal disorders(FGIDs)model rats,and investigate its mechanism. Methods:The FGIDs rat model was established by comprehensive method. EA at Zusanli(ST 36)of FGIDs model rats,and contrast with Yingu(KI10)point and non-meridian-non-acupoint. once a day,a total of 7 days of acupuncture. Simultaneously,the gastric and intestinal surface microcirculation blood flow was monitored by Laser Doppler flowmetry,and the change of 5-HT content in blood,hypothalamus,spinal cord,the stomach and intestines was detected through enzyme-linked immunosorbentassay. Results:The gastrointestinal microcirculation was obviously improved in Zusanli group(P<0.05),and the content of 5-HT was reduced in blood,hypothalamus,spinal cord,the stomach and intestines and so on all positions of the FGIDs model rats(P<0.05),and compared with the Yingu(KI10)group and the non-meridian-non-acupoint group,there was significant difference(P<0.05). Conclusion:FGIDs model rats’ gastrointestinal microcirculation status on the whole can be improved by EA at Zusanli(ST36),and the possible mechanism is related to the 5-HT content of all horizontal positions,and there is acupoint specificity.

关 键 词：功能性胃肠病 胃肠微循环 足三里穴 5-HT 

分 类 号：R245.97[医药卫生—针灸推拿学]