星点设计法优化地喹氯铵修饰紫杉醇与和厚朴酚共聚物胶束制备工艺  

Optimizing Prescription of Dequalinium Modified Paclitaxel and Honokiol Polymeric Micelles by Central Composite Design-Response Surface Methodology

作　　者：王鑫 肖瑶 符敏 刘晶晶 李学涛 程岚 WANG Xin;XIA0Yao;FU Min;LIU Jingjing;LI Xuetao;CHENG Lan(School of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,Liaoning,China)

机构地区：[1]辽宁中医药大学药学院

出　　处：《辽宁中医杂志》2018年第12期2608-2610,2703,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81673603)

摘　　要：目的:制备地喹氯铵修饰紫杉醇与和厚朴酚Soluplus/TPGS共聚物胶束,并通过星点设计-响应面法优选出最佳处方,提高难溶性药物紫杉醇与和厚朴酚的包封率。方法:采用薄膜水化法制备地喹氯铵修饰紫杉醇与和厚朴酚Soluplus/TPGS共聚物胶束。采用星点设计-响应面法并使用Design-Expert 8.0.6.1统计学软件对试验组的响应值进行回归分析,优化共聚物胶束的制备工艺条件。结果:共聚物胶束最佳制备工艺为Soluplus/紫杉醇=150:1(质量比),Soluplus/TPGS=8:1(质量比),水化温度25℃。胶束中紫杉醇与和厚朴酚含量的综合评分为96.11%。结论:星点设计-响应面法优选出的地喹氯铵修饰紫杉醇与和厚朴酚Soluplus/TPGS共聚物胶束的制备工艺条件的重复性良好,操作简便,所建模型具有较好的实用性和预测性。Objective:Dequalinium modified paclitaxel and honokiol Soluplus/TPGS polymeric micelles were prepared and the best prescription was optimized by central composite design-response surface methodology to increase the encapsulation efficiency of paclitaxel and honokiol.Methods:Dequalinium modified paclitaxel and honokiol Soluplus/TPGS polymeric micelles were prepared by film hydration method.The regression analysis of each experimental group was evaluated by central composite design-response surface methodology and the Design-Expert 8.0.6.1 software.Results:The best prescription was as following:Soluplus/paclitaxel=150:1(mass ratio);Soluplus/TPGS=8:1(mass ratio);the hydration temperature was 25 ℃ and the comprehensive score of encapsulation efficiencies was 96.11%.Conclusion:The preparation process optimized by central composite design-response surface methodology had a good repeatability and simple preparation,which indicated that this model possessed a good practicability and predictability.

关 键 词：共聚物胶束 星点设计-响应面法 紫杉醇 和厚朴酚 处方优选 

分 类 号：R284[医药卫生—中药学]