两种基因分型方法在河北省鼠疫耶尔森菌基因分型中的应用研究  

Studies on the genotypes of Yersinia pestis in Hebei province by two genotyping methods

作　　者：王海峰[1] 张懿晖[1] 杨晓燕[1] 刘溢洋[1] 牛艳芬[1] 刘广[1] WANG Hai-feng;ZHANG Yi-hui;YANG Xiao-yan;LIU Yi-yang;NIU Yan-fen;LIU Guang(Anti-plague Institute of Hebei Province,Zhangjiakou 075000,Hebei Province,China)

机构地区：[1]河北省鼠疫防治所检验科,河北张家口075000

出　　处：《中国媒介生物学及控制杂志》2018年第6期564-566,575,共4页Chinese Journal of Vector Biology and Control

基　　金：河北省2017年度医学科学研究重点课题计划(20170466)~~

摘　　要：目的分析河北省鼠疫疫源地分离的鼠疫耶尔森菌(鼠疫菌)基因型别和遗传变异特征。方法采取水煮法对康保县1972-2017年分离的鼠疫菌提取DNA,利用已有文献报道的多位点可变数目串联重复序列(MLVA)和差异区段(DFR)两种分型方法,经过PCR扩增,琼脂糖凝胶电泳和序列分析后确定被测菌株的基因型别。结果河北省分离的鼠疫菌MLVA基因分型一致,并确定了串联重复序列位点的重复数目;DFR基因型为G17和G20。G17型主要缺失DFR1、6、7、13、15、16、17、18,G20型主要缺失DFR1、6、7、12、13、15、16、17、18。结论河北省鼠疫疫源地分离的鼠疫菌株遗传特征比较稳定,2017年动物间疫情是邻近地区的疫情蔓延还是当地菌株的遗传变异,需要进一步研究。Objective To analyze the genotypes and genetic characteristics of Yersinia pestis isolated from Hebei plaguefoci. Methods The DNA of Y. pestis collected in Kangbao county from 1972 to 2017 was extracted by boiling method, themultiple locus variable number tandem repeat(MLVA) and the DFR primers according to a publication were synthesized bya biological company, then amplified by polymerase chain reaction, then genotypes were analyzed by agarose gelelectrophoresis. Results All of the strains showed one MLVA genotype, and the main genotypes of Y. pestis from Hebeifoci were G17 and G20. The absence of DFRs were 1, 6, 7, 13, 15, 16, 17, 18 in G17, the absence of DFRs were 1, 6, 7, 12,13, 15, 16, 17, 18 in G20. Conclusion The genetic characteristics of enzootic plague in Hebei province remain stable.Further studies are warranted to confirm whether the epidemics among wildlife in 2017 was caused by spread of neighboringfoci or by genetic shifting of the pathogen.

关 键 词：鼠疫耶尔森菌 差异区段 多位点串联重复序列 基因分型 

分 类 号：Q78[生物学—分子生物学] R378.61[医药卫生—病原生物学]