新疆乌苏市古尔图地区长尾黄鼠感染巴尔通体情况调查  

Analysis on Bartonella infection status of Citellus undulatus in Wusu city Guertu township, Xinjiang

作　　者：李博[1] 岳锡宏[1] 卢珊[2] 雒涛[1] 王信惠[1] 黎唯[1] LI Bo;YUE Xi-hong;LU Shan;LUO Tao;WANG Xin-hui;LI Wei(Xinjiang Center for Disease Control and Prevention,Urumqi 830002,Xinjiang Uygur Autonomous Region,China;National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention)

机构地区：[1]新疆维吾尔自治区疾病预防控制中心鼠疫防治科,乌鲁木齐830002 [2]中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《中国媒介生物学及控制杂志》2018年第6期567-570,共4页Chinese Journal of Vector Biology and Control

基　　金：中央财政行业专项(131031102000150003)~~

摘　　要：目的了解新疆维吾尔自治区(新疆)乌苏市古尔图地区长尾黄鼠的巴尔通体感染状况,为该地区鼠传疾病风险预警与防控措施制定提供科学依据。方法 2017年5-9月采用一日弓形夹法在查岗果勒、布兰布拉克、白石头等地捕获长尾黄鼠86只,无菌采集鼠体肾脏,试剂盒法提取全基因组DNA。应用实时荧光定量PCR法检测样本DNA巴尔通体特异性基因ssrA,计算感染率,分析样本的检测循环阈值(cp值)。结果 42只长尾黄鼠的巴尔通体实时荧光定量PCR检测为阳性,阳性率为48.84%;阳性样本cp值最低为27.49,最高为37.83,其中88.10%阳性样本的cp值在35.00～37.83之间,查岗果勒、布兰布拉克、白石头三地巴尔通体的阳性率分别为48.48%、60.87%和40.00%;布兰布拉克地区巴尔通体感染率、阳性动物染蚤率、蚤指数均高于查岗果勒和白石头地区;三地巴尔通体感染率与动物染蚤率差异无统计学意义(P>0.05)。结论新疆乌苏市古尔图地区长尾黄鼠间存在巴尔通体自然感染,传播范围广,但群体带菌量较低。Objective To understand the infection status of Bartonella in Citellus undulatus from Guertu,and to provide the basis for risk early warning and control measures of rodent-borne diseases.Methods In total,86 C.undulatus were collected from Chagangguole,Bulanbulake and Baishitou by bow-shaped clip method during 24 hours,the kidneys of the captured rodents were sampled under aseptic condition,genome DNA was extracted and detected by real-time fluorescence quantitative PCR targeting ssrA of Bartonella.The rate of infection were calculated and analyzed by cp test (cp value). Results A total of 86 C.undulatus were tested,the infection rate of Bartonella in those rodents was 48.84%(42/86).The cp of positive samples had the highest value of 37.83 and the lowest value of 27.49,88.10% of positive samples was in the "35.00-37.83"range.The infection rate of Chagangguole,Bulanlulake and Baishitou were 48.48%,60.87%,40.00%, respectively.The flea infection rate and flea index in Bulanlulake were higher than those of other places,the infection rate had no correlation with the flea infection in Guertu (P>0.05).Conclusion There were natural infection of Bartonella among C.undulatus in Guertu,with tendency of wide spread,but pathogen loads were relatively low in wild rodents.

关 键 词：巴尔通体 实时荧光定量PCR 古尔图地区 感染调查 

分 类 号：S443[农业科学—农业昆虫与害虫防治] Q939.93[农业科学—植物保护]