益气活血解毒中药对梗阻性肾病细胞自噬的调控作用  

Chinese Medicine of Yiqi Huoxue Jiedu Has Regulatory Effect on Cells Autophagy of Obstructive Nephropathy

作　　者：柴亚男 郝娟 马雪莲 闫翠环 王香婷 许庆友 CHAI Ya'nan;HAO Juan;MA Xuelian;YAN Cuihuan;WANG Xiangting;XU Qingyou

机构地区：[1]河北中医学院研究生学院,河北石家庄050091 [2]河北省肝肾病证研究重点实验室,河北石家庄050091

出　　处：《新中医》2018年第12期8-11,共4页New Chinese Medicine

基　　金：国家自然科学基金项目(81473652);河北省科技支撑项目(15277746D)

摘　　要：目的:观察益气活血解毒中药对梗阻性肾病中细胞自噬的作用。方法:将48只雄性Wistar大鼠随机分为假手术组、模型组、缬沙坦组和中药组,除假手术组外,其余各组结扎大鼠单侧输尿管复制梗阻性肾病的动物模型。缬沙坦组和中药组分别给予10 mg/(kg·d)缬沙坦和14 g/(kg·d)益气活血解毒中药,10天后摘取梗阻侧肾脏。通过Western blot和免疫组化检测血清和糖皮质激素诱导蛋白激酶(Serum and glucocorticoid induced kinase,SGK-1)、自噬相关基因5 (Autophagy associated gene 5,Atg5)、自噬相关基因Beclin-1、微管相关蛋白1轻链3 (Microtubular-associated protein 1 light chain 3,LC3)、磷酸化细胞外信号调节激酶1/2(Phosphorylated extracellular signal-regulated protein kinase,P-ERK1/2)、磷酸化哺乳动物雷帕霉素靶蛋白(Phosphorylated target of rapamycin,P-mTOR)的表达。结果:与假手术组比较,模型组自噬蛋白Atg5、Beclin-1、LC3Ⅱ/Ⅰ、SGK-1与P-ERK1/2表达增强,P-mTOR表达减弱,差异均有统计学意义(P <0.01)。与模型组比较,缬沙坦组和中药组自噬蛋白Atg5、Beclin-1、LC3Ⅱ/Ⅰ、SGK-1及P-ERK1/2表达降低,P-mTOR表达增强,差异均有统计学意义(P <0.05,P <0.01)。结论:益气活血解毒中药可以通过调控SGK-1、P-ERK1/2和P-mTOR通路,抑制盐皮质激素受体(Mineralocorticoid receptor,MR)的活化,从而纠正梗阻性肾病中细胞自噬的异常。Objective: To observed the effect of Chinese medicine of Yiqi Huoxue Jiedu on cells autophagy of obstructive nephropathy. Methods:Randomly divided 48 male Wistar rats into the sham operation group,the model group,the valsartan group and the Chinese medicine group. Except the sham operation group,the other groups received unilateral ureteral obstruction to copy the animal model of obstructive nephropathy. The valsartan group and the Chinese medicine group were given 10 mg/(kg·d) of valsartan and 14 g/(kg·d) of Chinese medicine of Yiqi Huoxue Jiedu,and obstructed kidney of rats were taken after 10 d. Detected the expressions of serum and glucocorticoid induced kinase (SGK-1),Autophagy associated gene 5 (Atg5),Beclin-1,microtubular-associated protein 1 light chain 3 (LC3), phosphorylated extracellular signal-regulated protein kinase (PERK1/2) and phosphorylated target of rapamycin (P-mTOR) by Western blot and immunohistochemistry. Results:Compared with those in the sham operation group,the expressions of Atg5,Beclin-1,LC3 II/I,SGK-1 and P-ERK1/2 in the model group were increased,while the expression of P-mToR was decreased, differences being significant(P<0.01). Compared with those in the model group,the expressions of Atg5,Beclin-1,LC3 II/I,SGK-1 and P-ERK1/2 in the valsartan group and the Chinese medicine group were decreased,while the expression of P-mToR was increased, differences being significant(P<0.05,P<0.01). Conclusion:Chinese medicine of Yiqi Huoxue Jiedu can inhibit the activation of mineralocorticoid receptor(MR) by regulating the pathways of SGK-1,ERK1/2 and P-mTOR,so as to correct the abnormal of cells autophagy of obstructive nephropathy.

关 键 词：梗阻性肾病 益气活血解毒 盐皮质激素受体(MR) 细胞自噬 血清糖皮质激素诱导蛋白激酶-1(SGK-1) 动物实验 大鼠 

分 类 号：R285.5[医药卫生—中药学]