补中益气汤对Ⅲ度脱垂性内痔组织中Fibulin-5表达影响的研究  

Study on Effect of Buzhong Yiqi Tang on Expression of Fibulin-5 in Grade-Ⅲ Prolapsed Hemorrhoid Tissues

作　　者：孙锋[1] 肖团有 陈辉 李玉英[1] SUN Feng;XIAO Tuanyou;CHEN Hui;LI Yuying

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]珠海市中西医结合医院,广东珠海519000 [3]广州市红十字会医院,广东广州510220

出　　处：《新中医》2018年第12期120-124,共5页New Chinese Medicine

基　　金：国家自然科学基金项目(81302976)

摘　　要：目的:探讨补中益气汤对Ⅲ度脱垂性内痔组织中Fibulin-5蛋白及其mRNA表达的影响。方法:将59例证型为脾虚气陷的Ⅲ度脱垂性内痔患者依门诊号顺序,按随机数字表法分为3组,其中治疗组(A组) 20例,对照组(B组) 20例,空白组(C组) 19例;A组病人口服草木犀流浸液片,同时服用补中益气汤;B组病人口服草木犀流浸液片;C组仅做空白对照,不服用任何药物,均治疗1周。疗程停止后第2天,分别于A、B、C组患者中收取肛垫组织标本各10例,重新对应分组为A1、B1、C1组,采用PCR、Western-blot技术从基因-蛋白层次系统研究比较各组患者内痔组织中Fibulin-5 mRNA与蛋白的表达分布差异。结果:Fibulin-5溶解曲线、β-Actin溶解曲线提示引物具有特异,且无杂带、无杂信号。3组间Fibulin-5 mRNA表达水平比较,差异有统计学意义(P <0.05)。组间的两两比较:A1组Fibulin-5 mRNA表达水平高于B1组(P <0.05);A1组Fibulin-5 mRNA表达水平高于C1组(P <0.05);B1组Fibulin-5 mRNA表达水平高于C1组(P <0.05)。从Fibulin-5蛋白条带发现,A1组中Fibulin-5蛋白表达最强且集中。3组间Fibulin-5蛋白表达水平比较,差异有统计学意义(F=12.581,P <0.05)。组间的两两比较:A1组Fibulin-5蛋白表达水平高于B1组(P <0.05);A1组Fibulin-5蛋白表达水平高于C1组(P <0.05);B1组与C1组Fibulin-5蛋白表达水平相当,差异无统计学意义(P> 0.05)。结论:补中益气汤可促进Ⅲ度脱垂性内痔组织中Fibulin-5蛋白及Fibulin-5mRNA的表达;Fibulin-5蛋白及Fibulin-5mRNA的表达水平的降低,可能是脱垂性内痔形成的病理学机制之一。Objective:To explore the effect of Buzhong Yiqi tang on the expression of protein and mRNA of Fibulin-5 in grade-Ⅲ prolapsed hemorrhoid tissues of patients. Methods: Divided 59 cases of patients with grade-Ⅲ prolapsed hemorrhoid who were differentiated with syndrome of sinking of qi due to spleen deficiency in outpatient number order into three groups according to random number table,20 cases in the treatment group (Group A),20 cases in the control group (Group B),and 19 cases in the blank group (Group C). Group A was treated with melilotus extract tablets orally and Buzhong Yiqi tang;Group B was simply treated with oral administration of melilotus extract tablets;Group C was given no medication. All groups were treated for one week. On the second day after treatment, respectively selected 10 cases of hemorrhoid tissue samples of patients and divided them into three groups, namely Group A1,B1 and C1. Adopted PCR and Western-blot technology to systematically study and compare the difference of the expression of protein and mRNA of Fibulin-5 in the hemorrhoid tissues of patients in each group from the gene-protein levels. Results: The Fibulin-5 dissolution curve and the β-Actin dissolution curve indicated that the primer was specific and had no stray band or noise signal. Compared the expression level of mRNA of Fibulin-5 between the three groups, the difference was significant(P<0.05). In pairwise comparison between groups,the expression level of mRNA of Fibulin-5 in Group A1 was higher than that in Group B1(P <0.05); the one in Group A1 was higher than that in Group C1(P<0.05);the one in Group B1 was higher than that in Group C1(P < 0.05). From the protein band of Fibulin-5,it was found that the expression of protein of Fibulin-5 was the strongest and concentrated in Group A1(P<0.05). Compared the expression level of protein of Fibulin-5 between the three groups,the difference was significant(F = 12.581,P = 0.000 < 0.05). In pairwise comparison between groups,the expression level of protein of Fibulin-5 in G

关 键 词：脱垂性内痔 Ⅲ度脱垂 补中益气汤 FIBULIN-5 

分 类 号：R657.18[医药卫生—外科学]