心痛泰对动脉粥样硬化兔主动脉NF-κB表达的影响  

Effect of Xintongtai on the expression of nuclear factor-kappa B in the aorta of rabbits with atherosclerosis

作　　者：任欣[1] 郭志华[2] REN Xin;GUO Zhi-hua(The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,Hunan,China;Hunan University of Chinese Medicine,Changsha 410208,Hunan,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医杂志》2018年第11期126-129,共4页Hunan Journal of Traditional Chinese Medicine

基　　金：湖南省中医药科研项目(编号:201516)

摘　　要：目的:观察心痛泰对动脉粥样硬化(AS)兔主动脉组织病理学改变和主动脉组织NF-κB表达的影响。方法:将120只新西兰大耳白兔随机分成6组,分别为正常对照组、模型组(均灌胃等体积0. 9%氯化钠注射液),心痛泰低、中、高剂量组(分别灌胃心痛泰2. 3g/kg、4. 6g/kg、9. 2g/kg),阿托伐他汀钙组(灌胃阿托伐他汀钙1. 1mg/kg),每组20只。通过高脂饲料喂养及免疫反应损伤法制备兔AS模型,造模的同时给予药物干预。8周后,光学显微镜观察兔主动脉组织病理学改变;采用Western bolt法分别检测兔主动脉组织细胞核及细胞浆中NF-κB的表达;采用RT-PCR法检测兔主动脉组织NF-κB m RNA的表达。结果:与模型组比较,各用药组主动脉内膜厚度变薄,内膜下泡沫细胞及炎性细胞的浸润均有不同程度的减轻,主动脉NF-κB核转位及NF-κB m RNA明显降低(P <0. 01或P <0. 05)。结论:心痛泰对AS兔主动脉组织有一定的保护作用,通过下调AS兔主动脉组织NF-κB核转位和NF-κB m RNA表达,抑制炎症反应,从而延缓动脉粥样硬化的形成。Objective :To investigate the effect of Xintongtai on pathologickl changes of the aorta and the expression of nuclear factor-kappa B (NF-κB)in the aorta in rabbits with atherosclerosis (AS).Methods:A total of 120New Zealand big-eared white rabbits were randomly divided into normal control group,model group (treated with an equal volume of 0.9%sodium chloride injection by gavage),low ,middle ,and high -dose Xintongtai groups (treated with Xintongtai at a dose of 2.3,4.6,and 9.2g/kg ,respectively,by gavage),and atorvastatin calcium group(treated with atorvastatin calcium at a dose of 1.1mg/kg by gavage),with 20rabbits in each group.A rabbit model of AS was established by high -fat diet and immunoreactive injury,and drug intervention was given during modeling.After 8weeks,a light microscope was used to observe the pathological changes of the aorta ;West- ern blot was used to measure the expression of NF-κB in nuclei and cytoplasm in the aorta ;RT -PCR was used to measure the mRNA expression of NF -κB in the aorta.Results :Compared with the model group,each administration group had a significant reduction in intimal thickness of the aorta,varying degrees of infiltration of foam ceils and inflammatory cells in the intima,and significant reductions in nuclear translocation and mRNA expression of NF-κB in the aorta(P <0.01or P <0.05).Conclusion:Xintongtai has a certain protective effect on the aorta of rabbits with AS and can delay the formation of AS by downregulating the nuclear translocation and mRNA expres- sion of NF -κB and inhibiting inflammatory response.

关 键 词：动脉粥样硬化 心痛泰 炎症反应 NF-ΚB 实验研究 

分 类 号：R285.5[医药卫生—中药学]