春兰DROOPING LEAF基因的克隆和表达分析  

Cloning and Expression Analysis of DROOPING LEAF Gene from Cymbidium goeringii

作　　者：刘换换 向林[3] 何国仁 陈跃[2] 孙崇波[2] 胡凤荣[1] Liu Huanhuan;Xiang Lin;He Guoren;Chen Yue;Sun Chongbo;Hu Fengrong(Nanjing Forestry University, Nanjing,210037;Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou,310021;Huazhong Agricultural University, Wuhan,430070)

机构地区：[1]南京林业大学,南京210037 [2]浙江省农业科学院园艺研究所,杭州310021 [3]华中农业大学,武汉430070

出　　处：《分子植物育种》2018年第23期7613-7619,共7页Molecular Plant Breeding

基　　金：林业公益性行业科研专项(201304117);国家青年自然科学基金(31300583;31400593);浙江省公益技术研究农业项目(2014C32100)共同资助

摘　　要：YABBY家族基因为种子植物所特有,参与胚胎发育、侧生器官分化等生物学过程,其主要成员之一DROOPING LEAF基因调控植物中脉的形成、心皮的发育。为了研究春兰DL基因的序列特征、表达特性,预测该基因在春兰花器官发育的作用,本研究从春兰中克隆1个DROOPING LEAF基因,命名为CgDL1,基因登录号为HM106986.1。序列分析表明,CgDL1基因含570bp长的开放阅读框,共编码189个氨基酸,分子量为21.386kD,预测等电点为9.11。氨基酸同源序列以及系统进化分析表明,CgDL1与其它植物的已知YABBY家族基因具有较高的同源性,与LiYABBY基因进化关系最为相近,属于DL/CRC亚族。实时荧光定量表达分析显示,CgDL1基因在春兰花器官中特异表达,在唇瓣中大量表达,侧瓣、萼片次之,在合蕊柱中几乎不表达。本研究为进一步探索YABBY家族在春兰花器官发育进程的调控作用及分子机理提供科学依据。The YABBY family gene is plant-specific involved in biological processes, including embryonic development and lateral organs differentiation. DROOPING LEAF gene plays an important role in the formation of leaf midribs and the development of carpel.In order to study gene sequence and expression characterization, and predict its role in organ development of Cymbidium goeringii,a DROOPING LEAF gene was cloned from C.goeringii named CgDL1 in this study (GenBank accession number HM106986.1).Sequence analysis showed that CgDL1 gene contained an open reading frame of 570 bp, encoding 189 amino acids with a molecular weight of 21.386 kD and a isoelectric point of 9.11. The amino acid homology sequence and phylogenetic analysis demonstrated that CgDL1 had high homology with other known YABBY proteins from different plants, which was most closely related with the evolution of LiYABBY gene and belonged to the CRC/DL subfamily. Real-time quantitative PCR showed that CgDL1 gene was specifically expressed in the organs, and was highly expressed in the lip, followed by lateral petals and sepals, and was weakly expressed in the column of C. goeringii. This study would set a basis for further exploring the regulatory role and molecular mechanism of the YABBY family during development of organs of C. goeringii.

关 键 词：YABBY家族 春兰 侧生器官 花器官发育 

分 类 号：S682.31[农业科学—观赏园艺]